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American Journal of Pathology, Vol 103, 70-79, Copyright © 1981 by American Society for Investigative Pathology

REGULAR ARTICLES

1-O-Alkyl-sn-glyceryl-3-phosphorylcholines: a novel class of neutrophil stimulants

JT O'Flaherty, RL Wykle, CH Miller, JC Lewis, M Waite, DA Bass, CE McCall and LR DeChatelet

1-O-Alkyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine aggregates and degranulates platelets and polymorphonuclear neutrophils. Here, the bioactivities of this platelet-activating factor, its 2-O-ethyl, and its 2-lyso derivatives were examined further. Each phospholipid aggregated and degranulated rabbit platelets and neutrophils with relative potencies of about 10,000 1,000, and 1, respectively. For rabbit neutrophils, and 2-O-acetyl compound was active in nanomolar and lower concentrations; required extracellular calcium and magnesium in order to induce aggregation; and required extracellular calcium and cytochlasin B in order to induce optimal degranulation. Furthermore the 2-O-acetyl and 2-O-ethyl compounds, in concentrations about tenfold higher than those required for rabbit neutrophils, aggregated and degranulated human neutrophils. With reference to these human neutrophil responses, degranulation required, and aggregation was dramatically enhanced by, cytochalasin B. The lysoanalog was unable to induce these response in the human cells. Thus, these lipids represent a novel class of neutrophil stimulants that closely resemble certain chemotactic factors (eg, C5a and synthetic oligopeptides) in their ability to aggregate and degranulate neutrophils and in the influences which calcium, magnesium and cytochalasin B have on their bioactions. Because platelet-activating factor circulates in the blood of rabbits and, perhaps, humans during anaphylaxis and is suspected of being involved in other syndromes such as serum sickness, this lipid may have unique biologic significance: it may act to recruit platelets and neutrophils into the lesions of these and similar pathologic syndromes.

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