This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jones, T. R. Articles by Bigner, D. D. Search for Related Content PubMed PubMed Citation Articles by Jones, T. R. Articles by Bigner, D. D.

American Journal of Pathology, Vol 103, 304-308, Copyright © 1981 by American Society for Investigative Pathology

REGULAR ARTICLES

Endothelial cell surface expression and binding of factor VIII/von Willebrand factor

TR Jones, KJ Kao, SV Pizzo and DD Bigner

Factor VIII/von Willebrand factor (FVIII/vWF), a glycoprotein molecular complex found in human plasma, has been demonstrated by cell membrane fluorescence to be present on the surface of cultured human umbilical cord vein endothelial cells. The endothelial nature of these cells was established by electron-microscopic studies that revealed the presence of Weibel-Palade bodies in virtually all cells cultured. A newly developed radioreceptor assay was used to detect FVIII/vWF in the medium taken from these endothelial cell cultures; FVIII/vWF concentration in the medium samples increased with time in culture. FVIII/vWF binding studies showed no significant FVIII/vWF-specific binding to endothelial cell surfaces and did not corroborate a previous report suggesting a FVIII/vWF-specific receptor on human umbilical cord vein endothelium. The presence of FVIII/vWF on endothelial cell membranes and the lack of receptor-mediated binding suggests that the FVIII/vWF either has been absorbed non-specifically to the cell surface or is an integral part of the endothelial cell membrane.

This article has been cited by other articles:

				K. Elvevold, B. Smedsrod, and I. Martinez The liver sinusoidal endothelial cell: a cell type of controversial and confusing identity Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G391 - G400. [Abstract] [Full Text] [PDF]

				M. J. Fontaine, J. Blanchard, C. Rastellini, V. Lazda, K. C. Herold, and R. Pollak Pancreatic Islets Activate Portal Vein Endothelial Cells In Vitro Ann. Clin. Lab. Sci., October 1, 2002; 32(4): 352 - 362. [Abstract] [Full Text] [PDF]

				M. Fukuchi, J. Watanabe, K. Kumagai, Y. Katori, S. Baba, K. Fukuda, T. Yagi, A. Iguchi, H. Yokoyama, M. Miura, et al. Increased von Willebrand factor in the endocardium as a local predisposing factor for thrombogenesis in overloaded human atrial appendage J. Am. Coll. Cardiol., April 1, 2001; 37(5): 1436 - 1442. [Abstract] [Full Text] [PDF]

				Y. Adler, K. Salozhin, M. Le Tonqueze, Y. Shoenfeld, and P. Youinou Review: Anti-Endothelial Cell Antibodies: a Need for Standardization Lupus, April 1, 1994; 3(2): 77 - 84. [Abstract] [PDF]