This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shtrasburg, S. Articles by Gal, R. Search for Related Content PubMed PubMed Citation Articles by Shtrasburg, S. Articles by Gal, R.

American Journal of Pathology, Vol 106, 141-144, Copyright © 1982 by American Society for Investigative Pathology

REGULAR ARTICLES

Demonstration of AA-protein in formalin-fixed, paraffin-embedded tissues

S Shtrasburg, M Pras, P Langevitch and R Gal

AA-protein was identified by SDS-acrylamide electrophoresis in amyloid fibrils fixed in formalin after isolation from fresh-frozen tissues obtained from patients with familial Mediterranean fever (FMF) amyloidosis and idiopathic AA-amyloidosis and, following deparaffination, rehydration and homogenization of embedded formalin- fixed tissues of old autopsy cases of the hereditary amyloidosis of FMF and amyloidosis acquired in association with tuberculosis, bronchiectasis, and rheumatoid arthritis. That AA-protein is unaltered by formalin was firmly established by agar gel diffusion using specific rabbit anti-AA serum. By contrast, AL proteins could not be demonstrated either in formalin-fixed amyloid fibrils derived from fresh-frozen tissues of a patient with presumably AL-amyloidosis dominated by cardiomegaly and one with AL-kappa amyloidosis or in blocks of cases of familial neuropathic amyloidosis, multiple myeloma, and idiopathic amyloidosis with cardiopathy. AA-protein is not denatured by formalin and retains its typical electrophoretic, chromatographic, and immunologic characteristics even 30 years after fixation and paraffin-embedding.

This article has been cited by other articles:

				S. Shtrasburg, R. Gal, E. Gruys, S. Perl, B. M. Martin, B. Kaplan, R. Koren, A. Nyska, M. Pras, and A. Livneh An Ancillary Tool for the Diagnosis of Amyloid A Amyloidosis in a Variety of Domestic and Wild Animals Vet. Pathol., February 1, 2005; 42(2): 132 - 139. [Abstract] [Full Text] [PDF]

				B Kaplan, S Shtrasburg, and M Pras Micropurification techniques in the analysis of amyloid proteins J. Clin. Pathol., February 1, 2003; 56(2): 86 - 90. [Abstract] [Full Text] [PDF]

				A Livneh, S Shtrasburg, B M Martin, J Baniel, R Gal, and M Pras Light chain amyloidosis of the urinary bladder. A site restricted deposition of an externally produced immunoglobulin J. Clin. Pathol., December 1, 2001; 54(12): 920 - 923. [Abstract] [Full Text] [PDF]