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American Journal of Pathology, Vol 111, 27-34, Copyright © 1983 by American Society for Investigative Pathology

REGULAR ARTICLES

Differential expression of a lamininlike substance by high- and low- metastatic tumor cells

J Varani, EJ Lovett 3d, JP McCoy Jr, S Shibata, DE Maddox, IJ Goldstein and M Wicha

High-metastatic murine fibrosarcoma cells readily attached to Type IV (basement membrane) collagen, whereas low-metastatic cells isolated from the same tumor did not. The addition of laminin--a glycoprotein that facilitates the adherence of epithelial cells to their basement membranes--enhanced the attachment of the low-metastatic cells, but not the high-metastatic cells. Using anti-laminin antibodies and a laminin- binding lectin as probes, the authors were able to identify by immunofluorescence a moiety associated with the high-metastatic cells, but not the low-metastatic cells, which cross-reacted with murine laminin purified from the EHS sarcoma. When extracts from the high- metastatic cells were separated by affinity chromatography, with the laminin-binding lectin as the affinity substrate, a substance was isolated that had an apparent molecular weight of 56,000 daltons. The affinity-purified material reacted strongly with anti-laminin antibodies by enzyme-linked immunosorbent assay.


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Copyright © 1983 by the American Society for Investigative Pathology.