help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oite, T.
Right arrow Articles by Vogt, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oite, T.
Right arrow Articles by Vogt, A.

American Journal of Pathology, Vol 112, 185-194, Copyright © 1983 by American Society for Investigative Pathology

REGULAR ARTICLES

An active model of immune complex glomerulonephritis in the rat employing cationized antigen

T Oite, F Shimizu, I Kihara, SR Batsford and A Vogt

An active model of in situ immune complex glomerulonephritis involving a cationic antigen was established. Three weeks after immunization with human IgG, the left kidneys of Wistar rats were perfused with 100 micrograms of cationized human IgG (pI greater than 9.5) via the left renal artery. At this time the animals exhibited a mean serum concentration of 0.58 +/- 0.33 mg anti-human IgG antibody per milliliter. Renal tissue was examined at regular intervals by immunofluorescence, light, and electron microscopy thereafter. Cationized human IgG and rat IgG were distributed along the glomerular capillary wall in a pattern that became increasingly granular with time; rat C3 was also present. Histologically, a severe proliferative lesion was seen with crescent formation in 10-20% of the glomeruli; adhesions of glomerular tufts to Bowman's capsule were common and with time spike formation in the glomerular basement membrane became very prominent. Extensive subepithelial dense deposits were seen by electron microscopy. Proteinuria was present in 19 of 26 animals within 24 hours, and in 30 of 31 by Day 7. Protein excretion fell from Day 14 onward, but some animals exhibited chronic proteinuria. The model described here represents another situation in which cationic antigens can induce subepithelial immune complexes, namely, the rapid release of small quantities of antigen into a previously sensitized host. This sequence could well mirror the events occurring in nature more closely than the previously described passive in situ model.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1983 by the American Society for Investigative Pathology.