| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 113, 198-206, Copyright © 1983 by American Society for Investigative Pathology
REGULAR ARTICLES |
RV Lloyd
Diethylstilbestrol (DES) treatment of weanling F344 female rats resulted in enlarged pituitary glands and diffuse pituitary prolactin (PRL) cell hyperplasia in all animals after 9 and 12 weeks of treatment. Serum PRL was significantly greater than in control rats (P less than 0.001). Immunohistochemical studies showed that most of the pituitary gland cells consisted of PRL cells. Ultrastructural studies showed increased numbers of PRL cells with hyperplasia of the rough endoplasmic reticulum and decreased numbers of secretory granules. There was a decrease in the relative number of growth hormone (GH) and other cell types in the anterior pituitary. Pituitary tumors and normal pituitary glands were dissociated with trypsin and maintained in culture for 3 weeks. The numbers of PRL and GH cells decreased with time in both groups, and there was an increase in the number of fibroblasts. Staining of the culture cells with neuron-specific enolase showed that the anterior pituitary cells were positive for this enzyme, while the fibroblastic cells were negative. When dissociated pituitary cells were cultured in the presence of 10(-9) M DES for 7 days, there was a 42% increase in the number of immunoreactive PRL cells. These results indicate that DES-treated rats provide an excellent model for study of the in vivo and in vitro regulation of pituitary hyperplasia and neoplasia.
This article has been cited by other articles:
 |
|
 |
|
  M. Ishida, W. Takahashi, S. Itoh, S. Shimodaira, S. Maeda, and J. Arita Estrogen Actions on Lactotroph Proliferation Are Independent of a Paracrine Interaction with Other Pituitary Cell Types: A Study Using Lactotroph-Enriched Cells Endocrinology, July 1, 2007; 148(7): 3131 - 3139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Pandey, A. Bannout, and D. L. Wendell The Edpm5 locus prevents the 'angiogenic switch' in an estrogen-induced rat pituitary tumor Carcinogenesis, October 1, 2004; 25(10): 1829 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Friedrichsen, S. Christ, H. Heuer, M. K. H. Schafer, A. F. Parlow, T. J. Visser, and K. Bauer Expression of Pituitary Hormones in the Pax8-/- Mouse Model of Congenital Hypothyroidism Endocrinology, March 1, 2004; 145(3): 1276 - 1283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Oomizu, K. Chaturvedi, and D. K. Sarkar Folliculostellate Cells Determine the Susceptibility of Lactotropes to Estradiol's Mitogenic Action Endocrinology, March 1, 2004; 145(3): 1473 - 1480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Gomez and J. A. Balsa Autocrine/Paracrine Action of Pituitary Vasoactive Intestinal Peptide on Lactotroph Hyperplasia Induced by Estrogen Endocrinology, October 1, 2003; 144(10): 4403 - 4409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hauspie, E. Seuntjens, H. Vankelecom, and C. Denef Stimulation of Combinatorial Expression of Prolactin and Glycoprotein Hormone {alpha}-Subunit Genes by Gonadotropin-Releasing Hormone and Estradiol-17{beta} in Single Rat Pituitary Cells during Aggregate Cell Culture Endocrinology, January 1, 2003; 144(1): 388 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Moons, S. Jirawatnotai, A. F. Parlow, G. Gibori, R. D. Kineman, and H. Kiyokawa Pituitary Hypoplasia and Lactotroph Dysfunction in Mice Deficient for Cyclin-Dependent Kinase-4 Endocrinology, August 1, 2002; 143(8): 3001 - 3008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cracchiolo, J. W. Swick, L. McKiernan, E. Sloan, S. Raina, C. Sloan, and D. L. Wendell Estrogen-Dependent Growth of a Rat Pituitary Tumor Involves, But Does Not Require, a High Level of Vascular Endothelial Growth Factor Experimental Biology and Medicine, July 1, 2002; 227(7): 492 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. V. Lloyd, K. H. Ruebel, S. Zhang, and L. Jin Pituitary Hyperplasia in Glycoprotein Hormone Alpha Subunit-, p18INK4C-, and p27kip-1-Null Mice : Analysis of Proteins Influencing p27kip-1 Ubiquitin Degradation Am. J. Pathol., March 1, 2002; 160(3): 1171 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Fitts, R. M. Klein, and C. A. Powers Estrogen and Tamoxifen Interplay with T3 in Male Rats: Pharmacologically Distinct Classes of Estrogen Responses Affecting Growth, Bone, and Lipid Metabolism, and Their Relation to Serum GH and IGF-I Endocrinology, October 1, 2001; 142(10): 4223 - 4235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. V. Shah, J. Chien, Y. P. Sun, S. Puri, and R. Ravindra Calcitonin Inhibits Anterior Pituitary Cell Proliferation in the Adult Female Rats Endocrinology, September 1, 1999; 140(9): 4281 - 4291. [Abstract] [Full Text]
|
|
|
|
|
|
T. J. Spady, K. L. Pennington, R. D. McComb, and J. D. Shull Genetic Bases of Estrogen-Induced Pituitary Growth in an Intercross between the ACI and Copenhagen Rat Strains: Dominant Mendelian Inheritance of the ACI Phenotype Endocrinology, June 1, 1999; 140(6): 2828 - 2835. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. L. Green, V. Speirs, A. M. Landolt, P. M. Foy, and S. L. Atkin 17{beta}-Hydroxysteroid Dehydrogenase Type 1, 2, 3, and 4 Expression and Enzyme Activity in Human Anterior Pituitary Adenomas J. Clin. Endocrinol. Metab., April 1, 1999; 84(4): 1340 - 1345. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. J. Spady, D. M. E. Harvell, A. Lemus-Wilson, T. E. Strecker, K. L. Pennington, E. A. Vander Woude, D. F. Birt, R. D. McComb, and J. D. Shull Modulation of Estrogen Action in the Rat Pituitary and Mammary Glands by Dietary Energy Consumption J. Nutr., February 1, 1999; 129(2): 587 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Asa and S. Ezzat The Cytogenesis and Pathogenesis of Pituitary Adenomas Endocr. Rev., December 1, 1998; 19(6): 798 - 827. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Shupnik, L. K. Pitt, A. Y. Soh, A. Anderson, M. B. Lopes, and E. R. Laws Jr. Selective Expression of Estrogen Receptor {alpha} and {beta} Isoforms in Human Pituitary Tumors J. Clin. Endocrinol. Metab., November 1, 1998; 83(11): 3965 - 3972. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Wynick, C. J. Small, A. Bacon, F. E. Holmes, M. Norman, C. J. Ormandy, E. Kilic, N. C. H. Kerr, M. Ghatei, F. Talamantes, et al. Galanin regulates prolactin release and lactotroph proliferation PNAS, October 13, 1998; 95(21): 12671 - 12676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Chaidarun, B. Swearingen, and J. M. Alexander Differential Expression of Estrogen Receptor-{beta} (ER{beta}) in Human Pituitary Tumors: Functional Interactions with ER{alpha} and a Tumor-Specific Splice Variant J. Clin. Endocrinol. Metab., September 1, 1998; 83(9): 3308 - 3315. [Abstract] [Full Text]
|
|
|
|
|
|
T.-Y. Chun, D. Gregg, D. K. Sarkar, and J. Gorski Differential regulation by estrogens of growth and prolactin synthesis in pituitary cells suggests that only a small pool of estrogen receptors is required for growth PNAS, March 3, 1998; 95(5): 2325 - 2330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Chaidarun, A. Klibanski, and J. M. Alexander Tumor-Specific Expression of Alternatively Spliced Estrogen Receptor Messenger Ribonucleic Acid Variants in Human Pituitary Adenomas J. Clin. Endocrinol. Metab., April 1, 1997; 82(4): 1058 - 1065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Gage, M. Roller, T. Saunders, L. Scarlett, and S. Camper Anterior pituitary cells defective in the cell-autonomous factor, df, undergo cell lineage specification but not expansion Development, January 1, 1996; 122(1): 151 - 160. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
