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American Journal of Pathology, Vol 114, 149-156, Copyright © 1984 by American Society for Investigative Pathology

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A microassay for colony-stimulating factor based on thymidine incorporation

MB Prystowsky, MF Naujokas, JN Ihle, E Goldwasser and FW Fitch

A variety of growth factors and lectins were tested; only colony- stimulating factors CSF-1, Interleukin 3, and a T-lymphocyte GM CSF induced colony formation in semisolid medium and stimulated thymidine incorporation in liquid culture. All other growth factors and lectins were inactive in both assays. Factor-stimulated thymidine incorporation was detectable 24 hours after stimulation and reached maximal levels 4- 6 days after stimulation. A convenient microassay for measuring CSF activity has been developed, enabling a large number of samples to be screened qualitatively in 2 days and permitting CSF activity to be measured quantitatively in 4-5 days. This microassay can supplement the clonal-cell assay method and be especially useful as an initial screening assay for CSF activity.


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E. Kawasaki, M. Ladner, A. Wang, J Van Arsdell, M. Warren, M. Coyne, V. Schweickart, M. Lee, K. Wilson, A Boosman, et al.
Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1)
Science, October 18, 1985; 230(4723): 291 - 296.
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Copyright © 1984 by the American Society for Investigative Pathology.