help button home button Am J Pathol Epitomics Buy 2 Antibodies Get 1 Free Special Offer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by White, J. G.
Right arrow Articles by Sauk, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by White, J. G.
Right arrow Articles by Sauk, J. J.

American Journal of Pathology, Vol 116, 514-522, Copyright © 1984 by American Society for Investigative Pathology

REGULAR ARTICLES

The organization of microtubules and microtubule coils in giant platelet disorders

JG White and JJ Sauk

Normal human platelets are characteristically discoid in shape. The lentiform appearance is supported by a circumferential band of microtubules lying just under the cell membrane along its greatest circumference. Some of the cells from patients with giant platelet disorders are also disk-shaped, but the majority of their huge platelets are spherical. In the present study platelets from patients with the Gray platelet syndrome (GPS), May-Hegglin anomaly (MHA), and Epstein's syndrome (ES) were examined in thin sections and negatively stained whole mounts, and by indirect immunofluorescence with a monoclonal antibody to tubulin for determination of the organization of their microtubule systems. Many GPS platelets and some ES and MHA platelets were discoid and contained circumferential bundles of microtubules. The number of coils in the band was increased 10-20-fold. Giant spherical platelets also contained increased numbers of individual microtubules and coils, but they were not organized into circumferential bundles. Immunofluorescence revealed an organization of assembled tubulin in the huge cells, suggestive of balls of yarn. Failure of the microtubules to organize into a circumferential band may explain why the majority of the huge cells have a spherical form.


This article has been cited by other articles:


Home page
 BloodHome page
R. Zunino, Q. Li, S. D. Rose, M. M. I. Romero-Benitez, T. Lejen, N. C. Brandan, and J.-M. Trifaro
Expression of scinderin in megakaryoblastic leukemia cells induces differentiation, maturation, and apoptosis with release of plateletlike particles and inhibits proliferation and tumorigenesis
Blood, October 1, 2001; 98(7): 2210 - 2219.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1984 by the American Society for Investigative Pathology.