help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Minta, J. O.
Right arrow Articles by Pambrun, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minta, J. O.
Right arrow Articles by Pambrun, L.

American Journal of Pathology, Vol 119, 111-126, Copyright © 1985 by American Society for Investigative Pathology


REGULAR ARTICLES

In vitro induction of cytologic and functional differentiation of the immature human monocytelike cell line U-937 with phorbol myristate acetate

JO Minta and L Pambrun

The tumor-promoting ester 4 beta-phorbol 12-myristate 13-acetate (PMA) has been shown to induce the differentiation of the immature monocytelike cell line U-937 c in vitro into a heterogeneous population of cells, including small "dense" cells, large vacuolized or "foamy" cells, spindle-shaped cells, and cells with multiple filopodia ("stellate" cells). The effect of PMA was dose- and time-dependent, the optimal conditions being 40-162 nM PMA for 48 hours. The minimum time of exposure to PMA to ensure further differentiation of U-937 cells was about 5 hours. The PMA-stimulated cells acquired morphologic, ultrastructural, and functional characteristics typical of cells of the monocyte/macrophage lineage. The PMA-treated U-937 cells became adherent, ceased to proliferate, and exhibited increased expression of monocyte-specific antigens (Leu-M2, - M3, HLADr), surface receptors (FcR, C3bR), enzymes (nonspecific esterase, transglutaminase), and ability to mediate chemotaxis, phagocytosis, superoxide anion production, and antibody-dependent cytotoxicity reactions. The induced cells lost their morphologic differentiation and ability to attach to surfaces and regained proliferative capacity upon repeated subculture in PMA-free media.


This article has been cited by other articles:


Home page
Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. H. P. Ong, Z. He, L. Kriazhev, X. Shan, R. G. E. Palfree, and A. Bateman
Regulation of progranulin expression in myeloid cells
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1602 - R1612.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
H. J. Knowles, D. R. Mole, P. J. Ratcliffe, and A. L. Harris
Normoxic Stabilization of Hypoxia-Inducible Factor-1{alpha} by Modulation of the Labile Iron Pool in Differentiating U937 Macrophages: Effect of Natural Resistance-Associated Macrophage Protein 1.
Cancer Res., March 1, 2006; 66(5): 2600 - 2607.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
I. M. Gavin, D. Glesne, Y. Zhao, C. Kubera, and E. Huberman
Spermine Acts as a Negative Regulator of Macrophage Differentiation in Human Myeloid Leukemia Cells
Cancer Res., October 15, 2004; 64(20): 7432 - 7438.
[Abstract] [Full Text] [PDF]


Home page
J. Exp. Med.Home page
L.-A. H. Allen, L. S. Schlesinger, and B. Kang
Virulent Strains of Helicobacter pylori Demonstrate Delayed Phagocytosis and Stimulate Homotypic Phagosome Fusion in Macrophages
J. Exp. Med., January 3, 2000; 191(1): 115 - 128.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
S. Kim, O. Bang, Y. Lee, and S. Kang
Production of inducible nitric oxide is required for monocytic differentiation of U937 cells induced by vitamin E-succinate
J. Cell Sci., January 2, 1998; 111(4): 435 - 441.
[Abstract] [PDF]


Home page
J. Biol. Chem.Home page
Z. Nemes Jr., R. Adany, M. Balazs, P. Boross, and L. Fesus
Identification of Cytoplasmic Actin as an Abundant Glutaminyl Substrate for Tissue Transglutaminase in HL-60 and U937 Cells Undergoing Apoptosis
J. Biol. Chem., August 15, 1997; 272(33): 20577 - 20583.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J.án Aragonés, C. López-Rodríguez, A. Corbí, P. Góm. del Arco, M. López-Cabrera, M. O. de Landázuri, and J. M. Redondo
Dithiocarbamates Trigger Differentiation and Induction of CD11c Gene through AP-1 in the Myeloid Lineage
J. Biol. Chem., May 3, 1996; 271(18): 10924 - 10931.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
S Higashiyama, J. Abraham, J Miller, J. Fiddes, and M Klagsbrun
A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF
Science, February 22, 1991; 251(4996): 936 - 939.
[Abstract] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the American Society for Investigative Pathology.