This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Salisbury, B. G. Articles by Minick, C. R. Search for Related Content PubMed PubMed Citation Articles by Salisbury, B. G. Articles by Minick, C. R.

American Journal of Pathology, Vol 120, 6-11, Copyright © 1985 by American Society for Investigative Pathology

REGULAR ARTICLES

Insoluble low-density lipoprotein-proteoglycan complexes enhance cholesteryl ester accumulation in macrophages

BG Salisbury, DJ Falcone and CR Minick

The interaction of arterial proteoglycans (PGs) and low-density lipoproteins (LDLs) has been postulated to be an important factor in extracellular cholesterol accumulation in the arterial wall. In the present study, insoluble complexes of LDL and PG (LDL-PG) were prepared and their effects on cholesteryl ester accumulation in mouse peritoneal macrophages was tested. The cholesteryl ester content of macrophages incubated with LDL-PG for 3 days was greater than 20 times that observed in cells incubated with LDL alone. The uptake of 125I-LDL by macrophages was markedly stimulated if LDL was incorporated into a complex with PG. However, in contrast to either LDL or acetylated LDL (ALDL), the extent of subsequent degradation of LDL-PG by the cells was reduced. The uptake and degradation of LDL-PG complexes stimulated macrophage incorporation of 14C-oleic acid into cholesteryl oleate 4- to 5-fold over LDL alone; however, esterification was significantly less than that observed with ALDL, even though more LDL-PG was degraded. Ultrastructurally, macrophages incubated with LDL-PG complexes contained lipid droplets as well as numerous phagocytic vacuoles often containing material similar in appearance to insoluble complexes. These results demonstrate that components of the extracellular matrix, such as PG, can modify the catabolism of LDL by scavenger cells. This phenomenon may be potentially important with respect to foam-cell genesis from macrophages in the arterial wall.

This article has been cited by other articles:

				E. E. Griffin, J. C. Ullery, B. E. Cox, and W. G. Jerome Aggregated LDL and lipid dispersions induce lysosomal cholesteryl ester accumulation in macrophage foam cells J. Lipid Res., October 1, 2005; 46(10): 2052 - 2060. [Abstract] [Full Text] [PDF]

				T. Kaji, A. Yamada, S. Miyajima, C. Yamamoto, Y. Fujiwara, T. N. Wight, and M. G. Kinsella Cell Density-dependent Regulation of Proteoglycan Synthesis by Transforming Growth Factor-beta 1 in Cultured Bovine Aortic Endothelial Cells J. Biol. Chem., January 14, 2000; 275(2): 1463 - 1470. [Abstract] [Full Text] [PDF]

				J. M. Manning, I. J. Edwards, W. D. Wagner, J. D. Wagner, M. R. Adams, and J. S. Parks Effects of Contraceptive Estrogen and Progestin on the Atherogenic Potential of Plasma LDLs in Cynomolgus Monkeys Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1216 - 1223. [Abstract] [Full Text]

				P. Vijayagopal and D. L. Glancy Macrophages Stimulate Cholesteryl Ester Accumulation in Cocultured Smooth Muscle Cells Incubated With Lipoprotein-Proteoglycan Complex Arterioscler. Thromb. Vasc. Biol., September 1, 1996; 16(9): 1112 - 1121. [Abstract] [Full Text]

				K. D. O'Brien, D. D. Reichenbach, S. M. Marcovina, J. Kuusisto, C. E. Alpers, and C. M. Otto Apolipoproteins B, (a), and E Accumulate in the Morphologically Early Lesion of `Degenerative' Valvular Aortic Stenosis Arterioscler. Thromb. Vasc. Biol., April 1, 1996; 16(4): 523 - 532. [Abstract] [Full Text]

				M. Kaartinen, A. Penttila, and P. T. Kovanen Extracellular Mast Cell Granules Carry Apolipoprotein B-100–Containing Lipoproteins Into Phagocytes in Human Arterial Intima : Functional Coupling of Exocytosis and Phagocytosis in Neighboring Cells Arterioscler. Thromb. Vasc. Biol., November 1, 1995; 15(11): 2047 - 2054. [Abstract] [Full Text]