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American Journal of Pathology, Vol 120, 244-247, Copyright © 1985 by American Society for Investigative Pathology

REGULAR ARTICLES

Ascorbate in aqueous humor protects against myeloperoxidase-induced oxidation

JT Rosenbaum, EL Howes Jr and D English

Chemotactic factors can cause polymorphonuclear leukocytes to release the contents of azurophilic granules, including the enzymes beta- glucuronidase and myeloperoxidase. In the presence of aqueous humor from the anterior chamber of the rabbit eye, the supernatant from stimulated leukocytes contains beta-glucuronidase, but myeloperoxidase is not detectable. Studies with aqueous humor and partially purified human myeloperoxidase suggest that this phenomenon is not due to a failure of enzyme release. The factor responsible for the inability to detect MPO in the assay system is heat-labile, dialyzable, and reversed by ascorbate oxidase. Comparable assay inhibition is produced by ascorbic acid at a concentration present in either human or rabbit aqueous humor. The ability of aqueous humor to protect against myeloperoxidase-induced oxidation may contribute to several diverse phenomena, including the susceptibility of the eye to Candida infection and a prolonged half-life for several inflammatory mediators in the anterior chamber.


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R. W. Welch, Y. Wang, A. CrossmanJr., J. B. Park, K. L. Kirk, and M. Levine
Accumulation of Vitamin C (Ascorbate) and Its Oxidized Metabolite Dehydroascorbic Acid Occurs by Separate Mechanisms
J. Biol. Chem., May 26, 1995; 270(21): 12584 - 12592.
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Copyright © 1985 by the American Society for Investigative Pathology.