This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Baron, R. Articles by Vignery, A. Search for Related Content PubMed PubMed Citation Articles by Baron, R. Articles by Vignery, A.

American Journal of Pathology, Vol 122, 363-378, Copyright © 1986 by American Society for Investigative Pathology

REGULAR ARTICLES

Kinetic and cytochemical identification of osteoclast precursors and their differentiation into multinucleated osteoclasts

R Baron, L Neff, P Tran Van, JR Nefussi and A Vignery

Positive identification of osteoclast percursors has not yet been possible. The authors have, in the present report, used a model system in the rat in which it is possible to induce the formation of multinucleated osteoclasts at a predictable and reproducible site and time (Tran Van P, Vignery A, Baron R. Anat Rec 1982, 202:445-451; Cell Tissue Res 1982, 225:283-292). This system allowed the investigation of the cellular events occurring locally during the recruitment and differentiation of osteoclast precursors. Prior to the formation of multinucleated osteoclasts, mononuclear cells positive for fluoride- inhibitable nonspecific esterase and cells positive for tartrate- resistant acid phosphatase increase in number locally. Double staining procedures demonstrated the presence of both enzymes in a number of cells, thereby suggesting that they are steps in the differentiation of a single cell population. Ultrastructural studies show that lysosomal enzymes are present in every compartment of the biosynthetic pathway, in small primary lysosomes and various forms of storage granules. As these precursors arrive at the bone surface, the storage granule lysosomes are markedly depleted. It is concluded that mononuclear precursors of the osteoclast are members of the mononuclear-phagocyte lineage and differentiate early to synthesize, store, and later secrete large quantities of lysosomal enzymes. The mature osteoclast, which, as its precursor, is positive for the mononuclear-phagocyte marker enzyme nonspecific esterase, results from the fusion of these mononuclear precursors, which occurs only after their attachment to the bone surface to be resorbed.

This article has been cited by other articles:

				T. Gattegno, A. Mittal, C. Valansi, K. C.Q. Nguyen, D. H. Hall, L. V. Chernomordik, and B. Podbilewicz Genetic Control of Fusion Pore Expansion in the Epidermis of Caenorhabditis elegans Mol. Biol. Cell, April 1, 2007; 18(4): 1153 - 1166. [Abstract] [Full Text] [PDF]

				I. Take, Y. Kobayashi, Y. Yamamoto, H. Tsuboi, T. Ochi, S. Uematsu, N. Okafuji, S. Kurihara, N. Udagawa, and N. Takahashi Prostaglandin E2 Strongly Inhibits Human Osteoclast Formation Endocrinology, December 1, 2005; 146(12): 5204 - 5214. [Abstract] [Full Text] [PDF]

				Y. Miura, M. Miura, S. Gronthos, M. R. Allen, C. Cao, T. E. Uveges, Y. Bi, D. Ehirchiou, A. Kortesidis, S. Shi, et al. Defective osteogenesis of the stromal stem cells predisposes CD18-null mice to osteoporosis PNAS, September 27, 2005; 102(39): 14022 - 14027. [Abstract] [Full Text] [PDF]

				Y. Kobayashi, I. Take, T. Yamashita, T. Mizoguchi, T. Ninomiya, T. Hattori, S. Kurihara, H. Ozawa, N. Udagawa, and N. Takahashi Prostaglandin E2 Receptors EP2 and EP4 Are Down-regulated during Differentiation of Mouse Osteoclasts from Their Precursors J. Biol. Chem., June 24, 2005; 280(25): 24035 - 24042. [Abstract] [Full Text] [PDF]

				Y. Kobayashi, T. Mizoguchi, I. Take, S. Kurihara, N. Udagawa, and N. Takahashi Prostaglandin E2 Enhances Osteoclastic Differentiation of Precursor Cells through Protein Kinase A-dependent Phosphorylation of TAK1 J. Biol. Chem., March 25, 2005; 280(12): 11395 - 11403. [Abstract] [Full Text] [PDF]

				W. ZOU, H. SCHWARTZ, S. ENDRES, G. HARTMANN, and Z. BAR-SHAVIT CpG oligonucleotides: novel regulators of osteoclast differentiation FASEB J, March 1, 2002; 16(3): 274 - 282. [Abstract] [Full Text] [PDF]

				X. Feng, S. L. Teitelbaum, M. E. Quiroz, S.-L. Cheng, C.-F. Lai, L. V. Avioli, and F. P. Ross Sp1/Sp3 and PU.1 Differentially Regulate beta 5 Integrin Gene Expression in Macrophages and Osteoblasts J. Biol. Chem., March 17, 2000; 275(12): 8331 - 8340. [Abstract] [Full Text] [PDF]

				X. Feng, S. L. Teitelbaum, M. E. Quiroz, D. A. Towler, and F. P. Ross Cloning of the Murine beta 5 Integrin Subunit Promoter. IDENTIFICATION OF A NOVEL SEQUENCE MEDIATING GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR-DEPENDENT REPRESSION OF beta 5 INTEGRIN GENE TRANSCRIPTION J. Biol. Chem., January 15, 1999; 274(3): 1366 - 1374. [Abstract] [Full Text] [PDF]

				T Laitala-Leinonen, C Lowik, S Papapoulos, and H. Vaananen Inhibition of intravacuolar acidification by antisense RNA decreases osteoclast differentiation and bone resorption in vitro J. Cell Sci., January 11, 1999; 112(21): 3657 - 3666. [Abstract] [PDF]

				P. A. Hill, A. Tumber, S. Papaioannou, and M. C. Meikle The Cellular Actions of Interleukin-11 on Bone Resorption in Vitro Endocrinology, April 1, 1998; 139(4): 1564 - 1572. [Abstract] [Full Text] [PDF]

				R. Jemtland, K. Lee, and G. V. Segre Heterogeneity Among Cells That Express Osteoclast-Associated Genes in Developing Bone Endocrinology, January 1, 1998; 139(1): 340 - 349. [Abstract] [Full Text] [PDF]

				N. A. ATHANASOU Current Concepts Review - Cellular Biology of Bone-Resorbing Cells J. Bone Joint Surg. Am., July 1, 1996; 78(7): 1096 - 1112. [Full Text]

				L Blavier and J. Delaisse Matrix metalloproteinases are obligatory for the migration of preosteoclasts to the developing marrow cavity of primitive long bones J. Cell Sci., January 12, 1995; 108(12): 3649 - 3659. [Abstract] [PDF]