help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kitamoto, T.
Right arrow Articles by Tateishi, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kitamoto, T.
Right arrow Articles by Tateishi, J.

American Journal of Pathology, Vol 123, 407-412, Copyright © 1986 by American Society for Investigative Pathology

REGULAR ARTICLES

Novel histochemical approaches to the prealbumin-related senile and familial forms of systemic amyloidosis

T Kitamoto, T Tashima and J Tateishi

The immunoperoxidase method, the autoclave method, and a newly developed alkaline-guanidine method were used to distinguish senile (SSA) and familial types (FAP) of prealbumin-related amyloidosis in formalin-fixed, paraffin-embedded tissue sections. Because all the amyloid deposits of SSA and FAP reacted positively with the antiprealbumin antiserum, a classification of the amyloid fibril proteins of FAP and SSA by immunohistochemistry, using polyclonal anti- prealbumin antisera, was not feasible. Both the senile and familial forms of amyloidosis showed unchanged Congophilia after prolonged autoclaving. In the alkaline-guanidine method, FAP amyloids were resistant to incubation for 2 hours. On the other hand, amyloid deposits of SSA lost the Congophilia and green birefringence with 2 hours' alkaline-guanidine treatment. Therefore, the autoclave method combined with the alkaline-guanidine method will considerably facilitate differentiation of SSA and FAP, without specific antisera.


This article has been cited by other articles:


Home page
 BrainHome page
M. Brett, M. R. Persey, M. M. Reilly, T. Revesz, D. R. Booth, S. E. Booth, P. N. Hawkins, M. B. Pepys, and J. A. Morgan-Hughes
Transthyretin Leu12Pro is associated with systemic, neuropathic and leptomeningeal amyloidosis
Brain, February 1, 1999; 122(2): 183 - 190.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Society for Investigative Pathology.