This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grone, H. J. Articles by Osborn, M. Search for Related Content PubMed PubMed Citation Articles by Grone, H. J. Articles by Osborn, M.

American Journal of Pathology, Vol 124, 294-302, Copyright © 1986 by American Society for Investigative Pathology

REGULAR ARTICLES

Villin--a marker of brush border differentiation and cellular origin in human renal cell carcinoma

HJ Grone, K Weber, U Helmchen and M Osborn

Expression of villin, a 95-kd F-actin bundling and severing protein, is restricted in animal tissues to epithelial cells with a brush border. Thus, the enterocytes of the intestine and epithelial cells of proximal but not distal tubules of the kidney are strongly positive. Here we report a similar staining pattern for human intestine and kidney. In four human colon adenocarcinomas villin expression was seen in tubular and glandular structures but not in the undifferentiated parts. Fourteen human renal carcinomas (9-Grade I and 5 Grade II) were villin- positive, and 2 sarcomatous renal carcinomas (Grade III) were villin- negative. The percentage of tumor cells that were villin-positive varied from 10-90% for the Grade I and II types. Our results indicate that villin may be a grading marker that deserves further study in renal carcinoma. They also raise the question whether the majority of renal carcinomas are derived from the proximal tubular epithelium rather than from the distal epithelium.

This article has been cited by other articles:

				K. Kimura, M. Iwano, D. F. Higgins, Y. Yamaguchi, K. Nakatani, K. Harada, A. Kubo, Y. Akai, E. B. Rankin, E. G. Neilson, et al. Stable expression of HIF-1{alpha} in tubular epithelial cells promotes interstitial fibrosis Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1023 - F1029. [Abstract] [Full Text] [PDF]

				S. P. George, Y. Wang, S. Mathew, K. Srinivasan, and S. Khurana Dimerization and Actin-bundling Properties of Villin and Its Role in the Assembly of Epithelial Cell Brush Borders J. Biol. Chem., September 7, 2007; 282(36): 26528 - 26541. [Abstract] [Full Text] [PDF]

				M. A. Esteban, M. G.B. Tran, S. K. Harten, P. Hill, M. C. Castellanos, A. Chandra, R. Raval, T. S. O'Brien, and P. H. Maxwell Regulation of E-cadherin Expression by VHL and Hypoxia-Inducible Factor. Cancer Res., April 1, 2006; 66(7): 3567 - 3575. [Abstract] [Full Text] [PDF]

				N. Kumar, P. Zhao, A. Tomar, C. A. Galea, and S. Khurana Association of Villin with Phosphatidylinositol 4,5-Bisphosphate Regulates the Actin Cytoskeleton J. Biol. Chem., January 23, 2004; 279(4): 3096 - 3110. [Abstract] [Full Text] [PDF]

				P. Marks, M Arai, J. Bandura, and D. Kwiatkowski Advillin (p92): a new member of the gelsolin/villin family of actin regulatory proteins J. Cell Sci., January 8, 1998; 111(15): 2129 - 2136. [Abstract] [PDF]

				D. Brown, R. Lee, and J. V. Bonventre Redistribution of villin to proximal tubule basolateral membranes after ischemia and reperfusion Am J Physiol Renal Physiol, December 1, 1997; 273(6): F1003 - F1012. [Abstract] [Full Text] [PDF]

				R Maunoury, S Robine, E Pringault, N Leonard, J. Gaillard, and D Louvard Developmental regulation of villin gene expression in the epithelial cell lineages of mouse digestive and urogenital tracts Development, January 7, 1992; 115(3): 717 - 728. [Abstract] [PDF]