help button home button Am J Pathol ASIP 2008 Summer Academy, Molecular Methcanisms of Human Disease: Injury, Inflammation, and Tissue Repair
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Craig, S. S.
Right arrow Articles by Schwartz, L. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Craig, S. S.
Right arrow Articles by Schwartz, L. B.

American Journal of Pathology, Vol 124, 427-435, Copyright © 1986 by American Society for Investigative Pathology

REGULAR ARTICLES

Mast cells in human keloid, small intestine, and lung by an immunoperoxidase technique using a murine monoclonal antibody against tryptase

SS Craig, G DeBlois and LB Schwartz

A murine monoclonal antibody (G5) against human lung mast cell tryptase was used for selective staining of human mast cells by an indirect immunoperoxidase method. Human tissues (keloid, small bowel, lung) were fixed in either Carnoy's fluid or neutral buffered formalin. In all three tissues the number and location of G5-stained mast cells corresponded closely with metachromatic toluidine blue-stained mast cells, although the immunospecific technique appeared to be more sensitive. In lung the average concentration of G5-positive mast cells after Carnoy's fixation was 15,695/cu mm of subepithelial tissue in bronchi and bronchioles and 26,580/cu mm of alveolar wall, in small bowel was 20,958/cu mm of mucosa and 8576/cu mm of submucosa, and in keloid was 3068/cu mm. Formalin fixation significantly reduced concentrations of G5-positive mast cells in all tissues except keloid.


This article has been cited by other articles:


Home page
 BloodHome page
D. Kempuraj, H. Saito, A. Kaneko, K. Fukagawa, M. Nakayama, H. Toru, M. Tomikawa, H. Tachimoto, M. Ebisawa, A. Akasawa, et al.
Characterization of Mast Cell-Committed Progenitors Present in Human Umbilical Cord Blood
Blood, May 15, 1999; 93(10): 3338 - 3346.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
T W Beer, H Baldwin, L West, P J Gallagher, and D H Wright
Mast cells in pathological and surgical scars
Br. J. Ophthalmol., June 1, 1998; 82(6): 691 - 694.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. W. Kofford, L. B. Schwartz, N. M. Schechter, D. R. Yager, R. F. Diegelmann, and M. F. Graham
Cleavage of Type I Procollagen by Human Mast Cell Chymase Initiates Collagen Fibril Formation and Generates a Unique Carboxyl-terminal Propeptide
J. Biol. Chem., March 14, 1997; 272(11): 7127 - 7131.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1986 by the American Society for Investigative Pathology.