This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fasth, A. Articles by Seiler, M. W. Search for Related Content PubMed PubMed Citation Articles by Fasth, A. Articles by Seiler, M. W.

American Journal of Pathology, Vol 125, 555-562, Copyright © 1986 by American Society for Investigative Pathology

REGULAR ARTICLES

Renal tubular immune complex formation in mice immunized with Tamm- Horsfall protein

A Fasth, JR Hoyer and MW Seiler

Mice were given injections of rat Tamm-Horsfall protein (TH) in order to study immune complex formation in the distal nephron. After immunization, all mice had high serum levels of antibodies to TH; immune deposits consisting of IgG antibodies to TH and TH formed at the base of cells of the thick ascending limb of the loop of Henle (ALH) and distal convoluted tubule. These basal complexes were maximal in number and size within the cortical ALH, where they often radiated toward the luminal surfaces. Although the highest anti-TH antibody titers were found in animals with the most extensive deposits, antibody levels were not directly proportional to IgG deposits or to the time after immunization. The larger immune deposits were visualized by light microscopy as PAS-positive deposits. The quantity of these deposits was proportional to the time after immunization and was directly related to anti-TH antibody levels. Electron microscopy showed that these immune deposits were present within the basal and lateral intercellular spaces of the cells of the ALH. The distribution and localization of TH within the normal mouse kidney was very similar to that in rats. However, the distribution of immune complexes within the distal nephron in mice differed from rats similarly treated with TH and indicates either species differences in the distribution and/or organization of TH associated with tubular cell surfaces or that the accessibility of distal tubules to antibodies is species-dependent.

This article has been cited by other articles:

				H. Tagawa, Y. Kizuka, T. Ikeda, S. Itoh, N. Kawasaki, H. Kurihara, M. L. Onozato, A. Tojo, T. Sakai, T. Kawasaki, et al. A Non-sulfated Form of the HNK-1 Carbohydrate Is Expressed in Mouse Kidney J. Biol. Chem., June 24, 2005; 280(25): 23876 - 23883. [Abstract] [Full Text] [PDF]

				M. Saemann, T Weichhart, M Zeyda, G Staffler, M Schunn, K. Stuhlmeier, Y Sobanov, T. Stulnig, S Akira, A von Gabain, et al. Immunoregulation in Urinary Tract Inflammation--A Role of Tamm-Horsfall Glycoprotein: Tamm-Horsfall Glycoprotein Links Innate Immune Cell Activation with Adaptive Immunity via a Toll-Like Receptor-4-Dependent Mechanism. J Clin Invest 115: 468-475, 2005 J. Am. Soc. Nephrol., April 1, 2005; 16(4): 829 - 836. [Full Text] [PDF]

				F. Lin, K. Cordes, L. Li, L. Hood, W. G. Couser, S. J. Shankland, and P. Igarashi Hematopoietic Stem Cells Contribute to the Regeneration of Renal Tubules after Renal Ischemia-Reperfusion Injury in Mice J. Am. Soc. Nephrol., May 1, 2003; 14(5): 1188 - 1199. [Abstract] [Full Text] [PDF]

				Y. Kiuchi-Saishin, S. Gotoh, M. Furuse, A. Takasuga, Y. Tano, and S. Tsukita Differential Expression Patterns of Claudins, Tight Junction Membrane Proteins, in Mouse Nephron Segments J. Am. Soc. Nephrol., April 1, 2002; 13(4): 875 - 886. [Abstract] [Full Text] [PDF]