This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nicosia, R. F. Articles by Madri, J. A. Search for Related Content PubMed PubMed Citation Articles by Nicosia, R. F. Articles by Madri, J. A.

American Journal of Pathology, Vol 128, 78-90, Copyright © 1987 by American Society for Investigative Pathology

REGULAR ARTICLES

The microvascular extracellular matrix. Developmental changes during angiogenesis in the aortic ring-plasma clot model

RF Nicosia and JA Madri

The composition of the extracellular matrix of developing microvessels in plasma clot cultures of rat aorta was studied with light and electron immunohistochemical techniques using affinity-purified antibodies against fibronectin, laminin, and collagen Types I, III, IV, and V. The extracellular matrix of solid endothelial sprouts in young cultures consisted of a delicate fibrillary network of fibronectin and Type V collagen and of patchy amorphous deposits of laminin and Type IV collagen. Rare fibrils of collagen Types I and III were also observed. Fibronectin stained intensely and appeared to be the predominant component of the provisional subendothelial matrix during vascular sprouting. As the cultures aged, laminin and Type IV collagen accumulated in the subendothelial space, forming a continuous feltwork around the newly formed microvessels. Patent microvessels were also surrounded by discontinuous deposits of fibronectin and by increased amounts of collagen Types I, III, and V. Ultrastructural studies revealed positive immunostaining for fibronectin, laminin, and collagen Types IV and V in the endoplasmic reticulum and in putative secretory vesicles, indicating active synthesis and secretion of these molecules by the endothelial cells. These observations indicate that the microvascular extracellular matrix undergoes significant dynamic changes during capillary development. The different composition and structural organization of the extracellular matrix at various stages of angiogenesis may have important effects on endothelial behavior and capillary morphogenesis.

This article has been cited by other articles:

				T.-H. Chun, F. Sabeh, I. Ota, H. Murphy, K. T. McDonagh, K. Holmbeck, H. Birkedal-Hansen, E. D. Allen, and S. J. Weiss MT1-MMP-dependent neovessel formation within the confines of the three-dimensional extracellular matrix J. Cell Biol., November 22, 2004; 167(4): 757 - 767. [Abstract] [Full Text] [PDF]

				K. Bein, E. T. Odell-Fiddler, and M. Drinane Role of TGF-{beta}1 and JNK signaling in capillary tube patterning Am J Physiol Cell Physiol, October 1, 2004; 287(4): C1012 - C1022. [Abstract] [Full Text] [PDF]

				C. Lugassy, H. K. Kleinman, J. A. Engbring, D. R. Welch, J. F. Harms, R. Rufner, G. Ghanem, S. R. Patierno, and R. L. Barnhill Pericyte-Like Location of GFP-Tagged Melanoma Cells: Ex Vivo and In Vivo Studies of Extravascular Migratory Metastasis Am. J. Pathol., April 1, 2004; 164(4): 1191 - 1198. [Abstract] [Full Text] [PDF]

				T. S. Hiran, J. E. Mazurkiewicz, P. Kreienberg, F. L. Rice, and S. E. LaFlamme Endothelial expression of the {alpha}6{beta}4 integrin is negatively regulated during angiogenesis J. Cell Sci., September 15, 2003; 116(18): 3771 - 3781. [Abstract] [Full Text] [PDF]

				S. Brodsky, J. Chen, A. Lee, K. Akassoglou, J. Norman, and M. S. Goligorsky Plasmin-dependent and -independent effects of plasminogen activators and inhibitor-1 on ex vivo angiogenesis Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1784 - H1792. [Abstract] [Full Text] [PDF]

				J. Chen, S. Brodsky, H. Li, D. J. Hampel, T. Miyata, T. Weinstein, U. Gafter, J. T. Norman, L. G. Fine, and M. S. Goligorsky Delayed branching of endothelial capillary-like cords in glycated collagen I is mediated by early induction of PAI-1 Am J Physiol Renal Physiol, July 1, 2001; 281(1): F71 - F80. [Abstract] [Full Text] [PDF]

				M. S. Goligorsky, J. Chen, and S. Brodsky Workshop: Endothelial Cell Dysfunction Leading to Diabetic Nephropathy : Focus on Nitric Oxide Hypertension, February 1, 2001; 37(2): 744 - 748. [Abstract] [Full Text] [PDF]

				K. L. Blackwell, Z. A. Haroon, S. Shan, W. Saito, G. Broadwater, C. S. Greenberg, and M. W. Dewhirst Tamoxifen Inhibits Angiogenesis in Estrogen Receptor-negative Animal Models Clin. Cancer Res., November 1, 2000; 6(11): 4359 - 4364. [Abstract] [Full Text] [PDF]

				K. J. Bayless, R. Salazar, and G. E. Davis RGD-Dependent Vacuolation and Lumen Formation Observed during Endothelial Cell Morphogenesis in Three-Dimensional Fibrin Matrices Involves the {alpha}v{beta}3 and {alpha}5{beta}1 Integrins Am. J. Pathol., May 1, 2000; 156(5): 1673 - 1683. [Abstract] [Full Text] [PDF]

				B. Kraling, D. Wiederschain, T Boehm, M Rehn, J. Mulliken, and M. Moses The role of matrix metalloproteinase activity in the maturation of human capillary endothelial cells in vitro J. Cell Sci., January 5, 1999; 112(10): 1599 - 1609. [Abstract] [PDF]

				M.-J. Lee, M. Evans, and T. Hla The Inducible G Protein-coupled Receptor edg-1 Signals via the G(i)/Mitogen-activated Protein Kinase Pathway J. Biol. Chem., May 10, 1996; 271(19): 11272 - 11279. [Abstract] [Full Text] [PDF]