This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Norton, A. J. Articles by Isaacson, P. G. Search for Related Content PubMed PubMed Citation Articles by Norton, A. J. Articles by Isaacson, P. G.

American Journal of Pathology, Vol 128, 225-240, Copyright © 1987 by American Society for Investigative Pathology

REGULAR ARTICLES

Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue

AJ Norton and PG Isaacson

Sixty-three well characterized B-cell lymphomas, with diagnoses previously established by conventional cryostat immunocytochemistry or limited paraffin immunocytochemistry, were studied. The tumors encompassed most of the Kiel subtypes and included the newly recognized entity, sclerosing mediastinal B-cell lymphoma. by the avidin-biotin peroxidase complex technique, each tumor was stained with a panel of monoclonal and polyclonal antibodies reactive in routinely fixed wax- embedded tissues. The panel included four reagents recognizing probable T-cell and B-cell restricted leukocyte common moieties (UCHL1, MT1, MB1, 4KB5), three antibodies to B-cell-related antigens (KiB3, MB2, LN1), and one to a macrophage-related antigen (Mac411). Other antibodies employed included anti-mu chain, anti-kappa, anti-lambda, and seven antibodies to non-phenotype-associated antigens, including HLA-DR (TAL-1B5, LN3, LN2, MB3), CD15 (C3D-1), and CD30 (BER-H2). Monotypic surface or perinuclear space and cytoplasmic immunoglobulin were detected in 80% of cases. Distinctive immunocytochemical profiles were demonstrable in many tumor categories by means of the panel of antibodies, thus facilitating the differential diagnosis of tumors of similar morphology. These results, together with our work on T-cell lymphoma in paraffin sections, show that accurate phenotypic analysis of lymphoma is now possible in routinely processed tissues.

This article has been cited by other articles:

				A. D. Attygalle, H. Liu, S. Shirali, T. C. Diss, C. Loddenkemper, H. Stein, A. Dogan, M.-Q. Du, and P. G. Isaacson Atypical marginal zone hyperplasia of mucosa-associated lymphoid tissue: a reactive condition of childhood showing immunoglobulin lambda light-chain restriction Blood, November 15, 2004; 104(10): 3343 - 3348. [Abstract] [Full Text] [PDF]

				T. C. Diss, H. Peng, A. C. Wotherspoon, L. Pan, P. M. Speight, and P. G. Isaacson A Single Neoplastic Clone in Sequential Biopsy Specimens from a Patient with Primary Gastric-Mucosa-Associated Lymphoid-Tissue Lymphoma and Sjogren's Syndrome N. Engl. J. Med., July 15, 1993; 329(3): 172 - 175. [Full Text]

				N. M. Smith, R. W. Byard, M. Vasiliou, D. F. Callen, A. J. Bourne, and A. S.-Y. Leong Pediatric Anaplastic Large Cell (CD30+) Lymphomas Associated With the t(2;5) (p23;q35) Chromosomal Abnormality International Journal of Surgical Pathology, July 1, 1993; 1(1): 43 - 49. [Abstract] [PDF]