This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vennegoor, C. Articles by Rumke, P. Search for Related Content PubMed PubMed Citation Articles by Vennegoor, C. Articles by Rumke, P.

American Journal of Pathology, Vol 130, 179-192, Copyright © 1988 by American Society for Investigative Pathology

REGULAR ARTICLES

A monoclonal antibody specific for cells of the melanocyte lineage

C Vennegoor, P Hageman, H Van Nouhuijs, DJ Ruiter, J Calafat, PJ Ringens and P Rumke
Division of Immunology, The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis), Amsterdam.

A monoclonal antibody, NKI/beteb, was prepared against membranes from a human melanoma metastasis, and in immunoprecipitates of melanoma cell lysates specific 100- and 7-kd glycoproteins were found. The large glycoproteins were also present in conditioned medium of melanoma cell lines. The antigen is located on the inner side of membranes of (pre)melanosomes and premelanosomelike vesicles. The antibody reacted in the immunoperoxidase test on frozen tissue sections with 27 of 28 nevocellular nevi (15/16 common, 12/12 dysplastic), 39/39 primary melanomas (3 intraepidermal, 24 cutaneous, 12 choroidal), 56/63 melanoma metastases, and 4/4 clear-cell sarcomas (melanoma of soft tissue). With sections of formalin-fixed paraffin-embedded tissues, the reaction was less sensitive. No reactivity was detected with frozen sections of 185 other tumors, except for 1 case of non-Hodgkin's lymphoma in which macrophages were positive. With the exception of melanocytes, all frozen sections of adult tissues that were tested were negative with NKI/beteb. On the basis of its tissue distribution so far, the antigen recognized by NKI/beteb seems to be a specific and sensitive diagnostic marker for cells of the melanocyte lineage.

This article has been cited by other articles:

				D. C. Harper, A. C. Theos, K. E. Herman, D. Tenza, G. Raposo, and M. S. Marks Premelanosome Amyloid-like Fibrils Are Composed of Only Golgi-processed Forms of Pmel17 That Have Been Proteolytically Processed in Endosomes J. Biol. Chem., January 25, 2008; 283(4): 2307 - 2322. [Abstract] [Full Text] [PDF]

				T. Hoashi, J. Muller, W. D. Vieira, F. Rouzaud, K. Kikuchi, K. Tamaki, and V. J. Hearing The Repeat Domain of the Melanosomal Matrix Protein PMEL17/GP100 Is Required for the Formation of Organellar Fibers J. Biol. Chem., July 28, 2006; 281(30): 21198 - 21208. [Abstract] [Full Text] [PDF]

				S. Kauser, A. Slominski, E. T. Wei, and D. J. Tobin Modulation of the human hair follicle pigmentary unit by corticotropin-releasing hormone and urocortin peptides FASEB J, May 1, 2006; 20(7): 882 - 895. [Abstract] [Full Text] [PDF]

				E. Bodo, T. Biro, A. Telek, G. Czifra, Z. Griger, B. I. Toth, A. Mescalchin, T. Ito, A. Bettermann, L. Kovacs, et al. A Hot New Twist to Hair Biology: Involvement of Vanilloid Receptor-1 (VR1/TRPV1) Signaling in Human Hair Growth Control Am. J. Pathol., April 1, 2005; 166(4): 985 - 998. [Abstract] [Full Text] [PDF]

				F. Levy, K. Muehlethaler, S. Salvi, A.-L. Peitrequin, C. K. Lindholm, J.-C. Cerottini, and D. Rimoldi Ubiquitylation of a Melanosomal Protein by HECT-E3 Ligases Serves as Sorting Signal for Lysosomal Degradation Mol. Biol. Cell, April 1, 2005; 16(4): 1777 - 1787. [Abstract] [Full Text] [PDF]

				S. Kauser, A. J. Thody, K. U. Schallreuter, C. L. Gummer, and D. J. Tobin A Fully Functional Proopiomelanocortin/Melanocortin-1 Receptor System Regulates the Differentiation of Human Scalp Hair Follicle Melanocytes Endocrinology, February 1, 2005; 146(2): 532 - 543. [Abstract] [Full Text] [PDF]

				A. Slominski, D. J. Tobin, S. Shibahara, and J. Wortsman Melanin Pigmentation in Mammalian Skin and Its Hormonal Regulation Physiol Rev, October 1, 2004; 84(4): 1155 - 1228. [Abstract] [Full Text] [PDF]

				J. F. Berson, A. C. Theos, D. C. Harper, D. Tenza, G. Raposo, and M. S. Marks Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis J. Cell Biol., May 12, 2003; 161(3): 521 - 533. [Abstract] [Full Text] [PDF]

				J. A. Ramos-Vara, M. A. Miller, G. C. Johnson, S. E. Turnquist, J. M. Kreeger, and G. L. Watson Melan A and S100 Protein Immunohistochemistry in Feline Melanomas: 48 Cases Vet. Pathol., January 1, 2002; 39(1): 127 - 132. [Abstract] [Full Text] [PDF]

				J. F. Berson, D. C. Harper, D. Tenza, G. Raposo, and M. S. Marks Pmel17 Initiates Premelanosome Morphogenesis within Multivesicular Bodies Mol. Biol. Cell, November 1, 2001; 12(11): 3451 - 3464. [Abstract] [Full Text] [PDF]

				J. A. Ramos-Vara, M. E. Beissenherz, M. A. Miller, G. C. Johnson, L. W. Pace, A. Fard, and S. J. Kottler Retrospective Study of 338 Canine Oral Melanomas with Clinical, Histologic, and Immunohistochemical Review of 129 Cases Vet. Pathol., November 1, 2000; 37(6): 597 - 608. [Abstract] [Full Text] [PDF]

				Y. Matsumoto, K. Horiba, J. Usuki, S. C. Chu, V. J. Ferrans, and J. Moss Markers of Cell Proliferation and Expression of Melanosomal Antigen in Lymphangioleiomyomatosis Am. J. Respir. Cell Mol. Biol., September 1, 1999; 21(3): 327 - 336. [Abstract] [Full Text]

				R. Golouh and A. Zidar Subungual Osteogenic Malignant Melanoma: A Case Report International Journal of Surgical Pathology, April 1, 1997; 4(4): 249 - 253. [Abstract] [PDF]