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American Journal of Pathology, Vol 130, 289-295, Copyright © 1988 by American Society for Investigative Pathology

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Partial characterization of a fibroblast-stimulating factor produced by cloned murine T lymphocytes

PJ Lammie, JG Monroe, AI Michael, GD Johnson, SM Phillips and MB Prystowsky
Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia.

T cells may regulate tissue fibrosis through the elaboration of soluble factors that stimulate fibroblast growth. The authors previously identified a factor produced by cloned Schistosoma mansoni antigen- specific T cells which served as a competence factor for murine fibroblasts. In the present report, they further characterize this fibroblast-stimulating factor (FsF) and differentiate it from a number of other T-cell-derived lymphokine activities. Crude supernatants from concanavalin-activated cloned T cells were fractionated by gel filtration, ion exchange, or reversed-phase high-pressure liquid chromatography. FsF has an apparent molecular weight of 17,000 and could be differentiated from colony-stimulating factor (CSF), interleukin-3 (IL-3), and interferon (IFN) on the basis of chromatographic characteristics. Highly purified or recombinant IL-2, IL-3, CSF, and IFN had no significant effect on fibroblast proliferation. Furthermore, a monoclonal anti-B-cell-stimulating factor- 1 antibody only partially blocked the fibroblast proliferation induced by T-cell supernatants.