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American Journal of Pathology, Vol 130, 505-514, Copyright © 1988 by American Society for Investigative Pathology

REGULAR ARTICLES

Correlation between differentiation and lung colonization by retinoic acid-treated F9 cells as revealed by the expression pattern of extracellular matrix and cell surface antigens [published erratum appears in Am J Pathol 1988 Jul;132(1):12]

L Leoncini, L Pacenti, D Rusciano, D Burroni, S Garbisa, M Cintorino and B Terrana
Laboratorio Biologia Cellulare, Centro Ricerche, Sclavo SpA, Siena, Italy.

For study of the correlation between differentiation and organ colonization properties of tumor cells, F9 embryonal carcinoma (EC) cells were treated with retinoic acid, an inducer of differentiation; and their organ colonization pattern was assessed by the experimental metastasis assay. Untreated cells were found to colonize the liver, whereas treated cells colonized the lungs. This pattern held true when metastases were scored after spontaneous death or after a careful microscopic search for micrometastases. Histologic examination revealed that both the tumor nodules produced by the untreated and the treated cells had the characteristics of EC devoid of any evidence of differentiation. The immunohistochemical study of the expression of markers typical of embryonal carcinoma cells or of the extracellular matrix components laminin and collagen type IV, typical of differentiated cells, confirmed these results. However, the lack of expression of stage-specific embryonal antigen 1 (SSEA-1), a marker generally associated with the undifferentiated state, observed only in the tumors obtained after injection of treated cells, indicates that the lung nodules probably derive from cells that have responded to the induction in vitro but have dedifferentiated in vivo.




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Copyright © 1988 by the American Society for Investigative Pathology.