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American Journal of Pathology, Vol 131, 1-4, Copyright © 1988 by American Society for Investigative Pathology

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Detection of viral DNA by in situ hybridization using bromodeoxyuridine- labeled DNA probes

G Niedobitek, T Finn, H Herbst, G Bornhoft, J Gerdes and H Stein
Institute for Pathology, Freie Universitat Berlin, West Germany.

Detection of viral DNA in infected tissues is demonstrated by in situ nucleic acid hybridization employing bromodeoxyuridine (BrdUrd)-labeled probes. BrdUrd is used alternatively to biotinylated nucleotides in the preparation of DNA probes and, after hybridization, developed with a monoclonal anti-BrdUrd antibody and the alkaline phosphatase-anti- alkaline phosphatase technique. The sensitivity using both BrdUrd- and biotin-labeled probes is similar. Furthermore, the simultaneous detection of viral coinfections will be possible by means of this technique in conjunction with biotin-labeled DNA probes.




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Copyright © 1988 by the American Society for Investigative Pathology.