| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 131, 298-307, Copyright © 1988 by American Society for Investigative Pathology
REGULAR ARTICLES |
DG Remick, SW Chensue, JC Hiserodt, GI Higashi and SL Kunkel
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602.
Synchronous models of T-cell-mediated and foreign body granulomas were induced in mice by intravenous embolization of Schistosoma mansoni eggs and Sephadex beads, respectively. The authors then performed flow- cytometric analysis of lymphocytes from dispersed granulomas, spleens, and peripheral blood at 4, 8, 16, and 32 days corresponding to the induction, growth, and maintenance, and resolution of these lesions. Lymphocytes were identified on the basis of light scatter characteristics, and the nature of the cells was confirmed by cell sorting and electron-microscopic examination. Lymphocyte subpopulations were characterized with antibodies to lymphocyte surface markers, specifically Ig, Thy 1.2, Lyt 1, Lyt 2, and L3T4. Natural killer cells were identified with anti-asialo GM1. Egg-induced granulomas had more lymphocytes of all phenotypes at all time points. Surprisingly, there was a significant number of cells staining positive for asialo GM1. On Day 16 after embolization there was a greater percentage of helper T cells, as defined by positive staining with L3T4, in the egg model, compared with the bead model. There was no obvious shift of lymphocytes from either the blood or spleen into the granuloma. These data confirm the importance of T cells in the direct participation of granulomatous inflammation, and the large numbers of asialo GM1-positive cells suggest a role for natural killer cells.
This article has been cited by other articles:
 |
|
 |
|
  K. Kuwata, H. Watanabe, S.-Y. Jiang, T. Yamamoto, C. Tomiyama-Miyaji, T. Abo, T. Miyazaki, and M. Naito AIM Inhibits Apoptosis of T Cells and NKT Cells in Corynebacterium-Induced Granuloma Formation in Mice Am. J. Pathol., March 1, 2003; 162(3): 837 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. O'REGAN, J. M. HAYDEN, S. BODY, L. LIAW, N. MULLIGAN, M. GOETSCHKES, and J. S. BERMAN Abnormal Pulmonary Granuloma Formation in Osteopontin-deficient Mice Am. J. Respir. Crit. Care Med., December 15, 2001; 164(12): 2243 - 2247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Park, K. F. Hoffmann, A. W. Cheever, D. Amichay, T. A. Wynn, and J. M. Farber Patterns of Chemokine Expression in Models of Schistosoma mansoni Inflammation and Infection Reveal Relationships between Type 1 and Type 2 Responses and Chemokines In Vivo Infect. Immun., November 1, 2001; 69(11): 6755 - 6768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yoshida, H. Maruyama, T. Kumagai, T. Amano, F. Kobayashi, M. Zhang, K. Himeno, and N. Ohta Schistosoma mansoni infection cancels the susceptibility to Plasmodium chabaudi through induction of type 1 immune responses in A/J mice Int. Immunol., August 1, 2000; 12(8): 1117 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen and D. L. Boros Identification of the Immunodominant T Cell Epitope of p38, a Major Egg Antigen, and Characterization of the Epitope-Specific Th Responsiveness During Murine Schistosomiasis Mansoni J. Immunol., June 1, 1998; 160(11): 5420 - 5427. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
