help button home button Am J Pathol Epitomics Buy 2 Antibodies Get 1 Free Special Offer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gu, J.
Right arrow Articles by Kirsch, I. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gu, J.
Right arrow Articles by Kirsch, I. R.

American Journal of Pathology, Vol 132, 13-17, Copyright © 1988 by American Society for Investigative Pathology

REGULAR ARTICLES

A study of myc-related gene expression in small cell lung cancer by in situ hybridization

J Gu, RI Linnoila, NL Seibel, AF Gazdar, JD Minna, BJ Brooks, GF Hollis and IR Kirsch
NCI-Navy Medical Oncology Branch, Naval Hospital, Bethesda, Maryland.

The expression of myc-related genes (c-myc, N-myc, and L-myc) in small cell lung cancer (SCLC) was studied by RNA-RNA tissue in situ hybridization. The tissues investigated included cytospins of ten cell lines derived from patients with SCLC, four corresponding nude mouse xenografts from cell lines, and metastatic tumor tissue obtained by surgical biopsy and at autopsy. The probes were prepared as 35S labeled complementary RNA. The expression of each gene was demonstrated specifically by autoradiography in the cytoplasm of the neoplastic cell samples. The average levels of oncogene expression in each specimen corroborated previous data obtained by Northern blot assays. In addition, heterogeneity in gene expression from cell to cell in each sample was noted. This study represents the first attempt to demonstrate oncogene expression in lung cancer cell lines and tissues in situ, and confirms that the expression of these myc related genes can be seen in the primary tumor. The technique of RNA-RNA tissue in situ hybridization has great potential in answering fundamental questions of tumor cell heterogeneity and progression in SCLC. It should be useful in both prospective and retrospective studies.


This article has been cited by other articles:


Home page
 ANGIOLOGYHome page
J. Gu, M. D' Andrea, M. Seethapathy, C. McDonnell, and R. Cichon
Physical Overdistension Converts Ventricular Cardiomyocytes to Acquire Endocrine Property and Regulate Ventricular Atrial Natriuretic Peptide Production
Angiology, March 1, 1991; 42(3): 173 - 186.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Society for Investigative Pathology.