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American Journal of Pathology, Vol 132, 406-409, Copyright © 1988 by American Society for Investigative Pathology

REGULAR ARTICLES

Cytoplasmic transfer between endothelium and lymphocytes: quantitation by flow cytometry

EC Guinan, BR Smith, PF Davies and JS Pober
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts.

Interactions between human lymphocytes and vascular endothelial cells in coculture have been examined using a newly developed fluorescence flow cytometric technique. Endothelium and blood lymphocytes are capable of bidirectional transfer of a cytoplasmic fluorescent dye, suggesting a novel path of communication between circulating blood cells and the vessel wall. This method also may be applicable to the analysis of other heterocellular interactions.

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