This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Manetto, V. Articles by Gambetti, P. Search for Related Content PubMed PubMed Citation Articles by Manetto, V. Articles by Gambetti, P.

American Journal of Pathology, Vol 134, 505-513, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Selective presence of ubiquitin in intracellular inclusions

V Manetto, FW Abdul-Karim, G Perry, M Tabaton, L Autilio-Gambetti and P Gambetti
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.

The authors have shown previously that ubiquitin, a protein involved in the degradation of short-lived and abnormal proteins, is present in several cytoplasmic inclusions of neurons. This study used a library of antibodies to ubiquitin and immunohistochemically examined for the presence of ubiquitin in nonviral intracytoplasmic inclusions that form in different cell types under various pathologic conditions. Membrane- bound lysosomal and nonlysosomal inclusions such as those of storage disease, Russell bodies, alpha-1-antitrypsin and alpha-fetoprotein as well as nonmembrane-bound inclusions were examined. Ubiquitin epitopes were detected in some of the nonmembrane-bound inclusions only. The ubiquitin-containing inclusions were the Rosenthal fibers, Mallory bodies, Crooke bodies, Lafora bodies, amyloid bodies, and the giant axons of giant axonal neuropathy. Nemaline bodies and the inclusions of juvenile digital fibromatosis, both of which contain actin and actinbinding proteins, did not show immunoreaction. These findings, as well as those of the previous study, show that the presence of ubiquitin in cellular inclusions is selective. The ubiquitin-containing inclusions are not membrane bound; they are fibrillary and most contain also intermediate filament-related proteins. The role of ubiquitin in the formation of these inclusions remains to be elucidated.

This article has been cited by other articles:

				B. A. Valentine, T. H. Flint, and K. A. Fischer Ubiquitin Expression in Muscle from Horses with Polysaccharide Storage Myopathy Vet. Pathol., May 1, 2006; 43(3): 270 - 275. [Abstract] [Full Text] [PDF]

				E. Wozniak, J. McBride, D. DeNardo, R. Tarara, V. Wong, and B. Osburn Isolation and Characterization of an Antigenically Distinct 68-kd Protein from Nonviral Intracytoplasmic Inclusions in Boa Constrictors Chronically Infected with the Inclusion Body Disease Virus (IBDV: Retroviridae) Vet. Pathol., September 1, 2000; 37(5): 449 - 459. [Abstract] [Full Text] [PDF]