This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wetzels, R. H. Articles by Ramaekers, F. C. Search for Related Content PubMed PubMed Citation Articles by Wetzels, R. H. Articles by Ramaekers, F. C.

American Journal of Pathology, Vol 134, 571-579, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast

RH Wetzels, R Holland, UJ van Haelst, EB Lane, IM Leigh and FC Ramaekers
Department of Pathology, University Hospital, Nijmegen, The Netherlands.

Using immunohistochemistry, the distribution patterns of basement membrane components type VII collagen (monoclonal antibody LH7.2), type IV collagen, and laminin were investigated in normal and malignant human breast tissue and compared with that of keratin 14 (monoclonal antibody LL002), which is expressed only by the basal (myoepithelial) cells in the secretory epithelia of the mammary gland. In normal breast tissue as well as in intraductal carcinomas, a more or less continuous basement membrane was observed at the epithelial stromal interface. Unlike laminin and type IV collagen, type VII collagen was not detected in the basement membrane of blood vessels. The keratin 14 antibody stained the basal cell layer of normal ducts and ducts with in situ cancer. In 85% of the invasive carcinomas no basement membrane or basal cells were detected. In 13 cases, however, laminin, type IV collagen, and/or type VII collagen were detected around tumor nests and individual tumor cells. Five of these tumors also showed a positive reaction with the keratin 14 antibody. In five cases keratin 14 expression was found without detectable basement membrane components. It is concluded that 18 of 103 invasive ductal breast carcinomas examined in this study exhibit a basal cell phenotype as determined from the expression of keratin and the deposition of basement membrane components.

This article has been cited by other articles:

				E Korsching, S S Jeffrey, W Meinerz, T Decker, W Boecker, and H Buerger Basal carcinoma of the breast revisited: an old entity with new interpretations J. Clin. Pathol., May 1, 2008; 61(5): 553 - 560. [Abstract] [Full Text] [PDF]

				L Da Silva, C Clarke, and S R Lakhani Demystifying basal-like breast carcinomas J. Clin. Pathol., December 1, 2007; 60(12): 1328 - 1332. [Abstract] [Full Text] [PDF]

				J. B. Arnes, J.-S. Brunet, I. Stefansson, L. R. Begin, N. Wong, P. O. Chappuis, L. A. Akslen, and W. D. Foulkes Placental Cadherin and the Basal Epithelial Phenotype of BRCA1-Related Breast Cancer Clin. Cancer Res., June 1, 2005; 11(11): 4003 - 4011. [Abstract] [Full Text] [PDF]

				C. Jones, E. Ford, C. Gillett, K. Ryder, S. Merrett, J. S. Reis-Filho, L. G. Fulford, A. Hanby, and S. R. Lakhani Molecular Cytogenetic Identification of Subgroups of Grade III Invasive Ductal Breast Carcinomas with Different Clinical Outcomes Clin. Cancer Res., September 15, 2004; 10(18): 5988 - 5997. [Abstract] [Full Text] [PDF]

				Y. Li, B. Welm, K. Podsypanina, S. Huang, M. Chamorro, X. Zhang, T. Rowlands, M. Egeblad, P. Cowin, Z. Werb, et al. Evidence that transgenes encoding components of the Wnt signaling pathway preferentially induce mammary cancers from progenitor cells PNAS, December 23, 2003; 100(26): 15853 - 15858. [Abstract] [Full Text] [PDF]

				S.-K. Tai, O. J.-K. Tan, V. T.-K. Chow, R. Jin, J. L. Jones, P.-H. Tan, A. Jayasurya, and B.-H. Bay Differential Expression of Metallothionein 1 and 2 Isoforms in Breast Cancer Lines with Different Invasive Potential: Identification of a Novel Nonsilent Metallothionein-1H Mutant Variant Am. J. Pathol., November 1, 2003; 163(5): 2009 - 2019. [Abstract] [Full Text] [PDF]

				T. Gudjonsson, L. Ronnov-Jessen, R. Villadsen, F. Rank, M. J. Bissell, and O. W. Petersen Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition J. Cell Sci., January 1, 2002; 115(1): 39 - 50. [Abstract] [Full Text] [PDF]

				B. S. Nielsen, F. Rank, J. M. Lopez, M. Balbin, F. Vizoso, L. R. Lund, K. Dano, and C. Lopez-Otin Collagenase-3 Expression in Breast Myofibroblasts as a Molecular Marker of Transition of Ductal Carcinoma in Situ Lesions to Invasive Ductal Carcinomas Cancer Res., October 1, 2001; 61(19): 7091 - 7100. [Abstract] [Full Text] [PDF]

				M. Morifuji, S.’i. Taniguchi, H. Sakai, Y. Nakabeppu, and M. Ohishi Differential Expression of Cytokeratin after Orthotopic Implantation of Newly Established Human Tongue Cancer Cell Lines of Defined Metastatic Ability Am. J. Pathol., April 1, 2000; 156(4): 1317 - 1326. [Abstract] [Full Text] [PDF]