This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chaponnier, C. Articles by Gabbiani, G. Search for Related Content PubMed PubMed Citation Articles by Chaponnier, C. Articles by Gabbiani, G.

American Journal of Pathology, Vol 134, 597-603, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Gelsolin modulation in epithelial and stromal cells of mammary carcinoma

C Chaponnier and G Gabbiani
Department of Pathology, University of Geneva, Switzerland.

Gelsolin, a Ca2+-sensitive protein present in many mammalian cells, regulates actin filament length by multiple mechanisms. In tumor cells, increased amounts of F-actin without change in its total amount have been observed, suggesting a modification in the organisation of this protein. The authors have localized gelsolin, total actin, alpha-smooth muscle actin, and prekeratin in frozen sections of normal human breast and infiltrating duct carcinomas by immunohistochemistry. A positive staining for gelsolin was observed in normal epithelial and myoepithelial cells but not in stromal fibroblasts. In contrast, no staining for gelsolin was detectable in carcinomatous cells, with the exception of remaining myoepithelial cells; myofibroblasts of the stromal reaction displayed an intense positive reaction. The absence of gelsolin staining in the epithelial cells of breast carcinoma may reflect their dedifferentiation or proliferative and invasive activities. The appearance of gelsolin in stromal cells raises the question as to what is its function in this situation. The immunohistochemical detection of gelsolin may be a useful adjunct to the study of mammary gland epithelium malignant transformation.

This article has been cited by other articles:

				B. Yermen, A. Tomas, and P. A. Halban Pro-Survival Role of Gelsolin in Mouse {beta}-Cells Diabetes, January 1, 2007; 56(1): 80 - 87. [Abstract] [Full Text] [PDF]

				S. Clement, B. Hinz, V. Dugina, G. Gabbiani, and C. Chaponnier The N-terminal Ac-EEED sequence plays a role in {alpha}-smooth-muscle actin incorporation into stress fibers J. Cell Sci., April 1, 2005; 118(7): 1395 - 1404. [Abstract] [Full Text] [PDF]

				K. B. Glaser, J. Li, M. E. Aakre, D. W. Morgan, G. Sheppard, K. D. Stewart, J. Pollock, P. Lee, C. Z. O'Connor, S. N. Anderson, et al. Transforming Growth Factor {beta} Mimetics: Discovery of 7-[4-(4-Cyanophenyl)phenoxy]-Heptanohydroxamic Acid, a Biaryl Hydroxamate Inhibitor of Histone Deacetylase Mol. Cancer Ther., August 1, 2002; 1(10): 759 - 768. [Abstract] [Full Text] [PDF]

				A. D. Thor, S. M. Edgerton, S. Liu, D. H. Moore II, and D. J. Kwiatkowski Gelsolin as a Negative Prognostic Factor and Effector of Motility in erbB-2-positive Epidermal Growth Factor Receptor-positive Breast Cancers Clin. Cancer Res., August 1, 2001; 7(8): 2415 - 2424. [Abstract] [Full Text] [PDF]

				J.-W. Han, S. H. Ahn, S. H. Park, S. Y. Wang, G.-U. Bae, D.-W. Seo, H.-K. Kwon, S. Hong, H. Y. Lee, Y.-W. Lee, et al. Apicidin, a Histone Deacetylase Inhibitor, Inhibits Proliferation of Tumor Cells via Induction of p21WAF1/Cip1 and Gelsolin Cancer Res., November 1, 2000; 60(21): 6068 - 6074. [Abstract] [Full Text]

				M. Chellaiah, N. Kizer, M. Silva, U. Alvarez, D. Kwiatkowski, and K. A. Hruska Gelsolin Deficiency Blocks Podosome Assembly and Produces Increased Bone Mass and Strength J. Cell Biol., February 21, 2000; 148(4): 665 - 678. [Abstract] [Full Text] [PDF]

				C. Chew, J. Parente, X Chen, C Chaponnier, and R. Cameron The LIM and SH3 domain-containing protein, lasp-1, may link the cAMP signaling pathway with dynamic membrane restructuring activities in ion transporting epithelia J. Cell Sci., January 6, 2000; 113(11): 2035 - 2045. [Abstract] [PDF]

				P. A. Folger, W. J. Berg, Z. DeJesus, Y. Fong, and J. D. Pardee A Mammalian Severin Replaces Gelsolin in Transformed Epithelial Cells Cancer Res., October 1, 1999; 59(20): 5349 - 5355. [Abstract] [Full Text] [PDF]

				P. A. JANMEY The Cytoskeleton and Cell Signaling: Component Localization and Mechanical Coupling Physiol Rev, July 1, 1998; 78(3): 763 - 781. [Abstract] [Full Text] [PDF]

				P. D. Arora and C. A.G. McCulloch Dependence of Fibroblast Migration on Actin Severing Activity of Gelsolin J. Biol. Chem., August 23, 1996; 271(34): 20516 - 20523. [Abstract] [Full Text] [PDF]

				C. Vasconcellos, P. Allen, M. Wohl, J. Drazen, P. Janmey, and T. Stossel Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin Science, February 18, 1994; 263(5149): 969 - 971. [Abstract] [PDF]