This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kleppel, M. M. Articles by Michael, A. F. Search for Related Content PubMed PubMed Citation Articles by Kleppel, M. M. Articles by Michael, A. F.

American Journal of Pathology, Vol 134, 813-825, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Human tissue distribution of novel basement membrane collagen

MM Kleppel, PA Santi, JD Cameron, J Wieslander and AF Michael
Department of Laboratory Medicine, University of Minnesota Medical School, Minneapolis 55455.

The authors have defined the specificity of monoclonal antibodies to collagen fragments of basement membrane (BM) and have used these highly specific antibodies to study the human tissue distribution of two novel 28 kd noncollagenous (NC1) peptides (M28 , M28+) compared with those derived from type IV collagen (alpha 1[26 kd] and alpha 2[24 kd] NC1). A limited distribution of the 28 kd peptides was observed in specialized BM of the kidney, eye, cochlea, lung, and brain, whereas type IV collagen is found in all human BM. These novel peptides, which colocalize with each other, are found in BM that also contain type IV collagen but do not, in all cases, colocalize with type IV collagen. The presence of the 28 kd peptides in the BM of the kidney, cochlea, and eye is in keeping with abnormalities involving these components in BM of patients with Alport familial nephritis (FN), who frequently have hearing loss, anterior lenticonus and retinal flecks in addition to renal disease. These 28 kd peptides are distinct, biochemically and immunochemically, from the alpha 1 and alpha 2 chain NC1 peptides of type IV collagen, and represent either peptide fragments of genetically distinct BM collagen molecules or additional molecules originating from the same gene family as type IV collagen.

This article has been cited by other articles:

				A. Kabosova, D. T. Azar, G. A. Bannikov, K. P. Campbell, M. Durbeej, R. F. Ghohestani, J. C. R. Jones, M. C. Kenney, M. Koch, Y. Ninomiya, et al. Compositional Differences between Infant and Adult Human Corneal Basement Membranes Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 4989 - 4999. [Abstract] [Full Text] [PDF]

				J. Favor, C. J. Gloeckner, D. Janik, M. Klempt, A. Neuhauser-Klaus, W. Pretsch, W. Schmahl, and L. Quintanilla-Fend Type IV Procollagen Missense Mutations Associated With Defects of the Eye, Vascular Stability, the Brain, Kidney Function and Embryonic or Postnatal Viability in the Mouse, Mus musculus: An Extension of the Col4a1 Allelic Series and the Identification of the First Two Col4a2 Mutant Alleles Genetics, February 1, 2007; 175(2): 725 - 736. [Abstract] [Full Text] [PDF]

				A. F. Zehnder, J. C. Adams, P. A. Santi, A. G. Kristiansen, C. Wacharasindhu, S. Mann, R. Kalluri, M. C. Gregory, C. E. Kashtan, and S. N. Merchant Distribution of Type IV Collagen in the Cochlea in Alport Syndrome Arch Otolaryngol Head Neck Surg, November 1, 2005; 131(11): 1007 - 1013. [Abstract] [Full Text] [PDF]

				W. K. Bolton, L. Chen, T. Hellmark, J. Wieslander, and J. W. Fox Epitope Spreading and Autoimmune Glomerulonephritis in Rats Induced by a T Cell Epitope of Goodpasture's Antigen J. Am. Soc. Nephrol., September 1, 2005; 16(9): 2657 - 2666. [Abstract] [Full Text] [PDF]

				L. Heidet, D.-B. Borza, M. Jouin, M. Sich, M.-G. Mattei, Y. Sado, B. G. Hudson, N. Hastie, C. Antignac, and M.-C. Gubler A Human-Mouse Chimera of the {alpha}3{alpha}4{alpha}5(IV) Collagen Protomer Rescues the Renal Phenotype in Col4a3-/- Alport Mice Am. J. Pathol., October 1, 2003; 163(4): 1633 - 1644. [Abstract] [Full Text] [PDF]

				L. Heidet, E. M. H. F. Bongers, M. Sich, S.-Y. Zhang, C. Loirat, A. Meyrier, M. Broyer, G. Landthaler, B. Faller, Y. Sado, et al. In Vivo Expression of Putative LMX1B Targets in Nail-Patella Syndrome Kidneys Am. J. Pathol., July 1, 2003; 163(1): 145 - 155. [Abstract] [Full Text] [PDF]

				L Chen, N Miyamura, Y Ninomiya, and J T Handa Distribution of the collagen IV isoforms in human Bruch's membrane Br. J. Ophthalmol., February 1, 2003; 87(2): 212 - 215. [Abstract] [Full Text] [PDF]

				S. J. Harvey, R. Mount, Y. Sado, I. Naito, Y. Ninomiya, R. Harrison, B. Jefferson, R. Jacobs, and P. S. Thorner The Inner Ear of Dogs with X-Linked Nephritis Provides Clues to the Pathogenesis of Hearing Loss in X-Linked Alport Syndrome Am. J. Pathol., September 1, 2001; 159(3): 1097 - 1104. [Abstract] [Full Text] [PDF]

				P. Barsotti, A. O. Muda, G. Mazzucco, L. Massella, B. Basolo, M. De Marchi, G. Rizzoni, G. Monga, and T. Faraggiana Distribution of {{alpha}}-chains of type IV collagen in glomerular basement membranes with ultrastructural alterations suggestive of Alport syndrome Nephrol. Dial. Transplant., May 1, 2001; 16(5): 945 - 952. [Abstract] [Full Text] [PDF]

				S. Gunwar, F. Ballester, M. E. Noelken, Y. Sado, Y. Ninomiya, and B. G. Hudson Glomerular Basement Membrane. IDENTIFICATION OF A NOVEL DISULFIDE-CROSS-LINKED NETWORK OF alpha 3, alpha 4, AND alpha 5 CHAINS OF TYPE IV COLLAGEN AND ITS IMPLICATIONS FOR THE PATHOGENESIS OF ALPORT SYNDROME J. Biol. Chem., April 10, 1998; 273(15): 8767 - 8775. [Abstract] [Full Text] [PDF]

				D Cosgrove, D T Meehan, J A Grunkemeyer, J M Kornak, R Sayers, W J Hunter, and G C Samuelson Collagen COL4A3 knockout: a mouse model for autosomal Alport syndrome. Genes & Dev., December 1, 1996; 10(23): 2981 - 2992. [Abstract] [PDF]

				Y. Segal, L. Zhuang, E. Rondeau, J.-D. Sraer, and J. Zhou Regulation of the Paired Type IV Collagen Genes COL4A5 and COL4A6. ROLE OF THE PROXIMAL PROMOTER REGION J. Biol. Chem., April 6, 2001; 276(15): 11791 - 11797. [Abstract] [Full Text] [PDF]