This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ishimura, E. Articles by Kashgarian, M. Search for Related Content PubMed PubMed Citation Articles by Ishimura, E. Articles by Kashgarian, M.

American Journal of Pathology, Vol 134, 843-855, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Formation of extracellular matrix by cultured rat mesangial cells

E Ishimura, RB Sterzel, K Budde and M Kashgarian
Department of Medicine, Yale University School of Medicine, New Haven,CT 06510.

Formation of extracellular matrix (ECM) by mesangial cells (MCs) contributes to progressive glomerulosclerosis. The authors investigated the production and distribution of ECM constituents by cultured rat MCs, using immunocytochemistry and immunoelectron microscopy. Staining for all ECM constituents increased after serum feeding. Localization was strictly intracellular until confluency, when extracellular deposition of collagen IV and laminin appeared, followed by fibronectin and collagen III. In parallel, the intracellular staining for these proteins diminished markedly. Neither extracellular deposition nor intracellular loss was observed for collagen I and thrombospondin. On surfaces coated with collagen IV or laminin, extracellular deposition of ECM constituents clearly preceded confluency. These results indicate that synthesis of ECM constituents parallels MC growth, and that extracellular deposition of ECM occurs at cell-cell contact. Collagen IV or laminin secreted by MCs in the substratum accelerates production and facilitates secretion of other ECM constituents in an autocrine fashion.

This article has been cited by other articles:

				Y. Xing, S. H. Ganji, J. W. Noh, and V. S. Kamanna Cell density-dependent expression of EDG family receptors and mesangial cell proliferation: role in lysophosphatidic acid-mediated cell growth Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1250 - F1257. [Abstract] [Full Text] [PDF]

				M. Iwahashi, Y. Muragaki, A. Ooshima, and R. Nakano Decreased Type III and V Collagen Expression in Chorionic Villi of Hydatidiform Mole J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3346 - 3350. [Abstract] [Full Text] [PDF]

				T. NAITO, M. S. RAZZAQUE, A. NAZNEEN, D. LIU, H. NIHEI, T. KOJI, and T. TAGUCHI Renal Expression of the Ets-1 Proto-oncogene during Progression of Rat Crescentic Glomerulonephritis J. Am. Soc. Nephrol., December 1, 2000; 11(12): 2243 - 2255. [Abstract] [Full Text]

				S. Harendza, A. Schneider, U. Helmchen, and R. A. K. Stahl Extracellular matrix deposition and cell proliferation in a model of chronic glomerulonephritis in the rat Nephrol. Dial. Transplant., December 1, 1999; 14(12): 2873 - 2879. [Abstract] [Full Text] [PDF]

				T. Nagasaki, E. Ishimura, H. Koyama, A. Shioi, S. Jono, M. Inaba, T. Hasuma, M. Yokoyama, Y. Nishizawa, H. Morii, et al. {alpha}v Integrin regulates TNF-{alpha}-induced nitric oxide synthesis in rat mesangial cells—possible role of osteopontin Nephrol. Dial. Transplant., August 1, 1999; 14(8): 1861 - 1866. [Abstract] [Full Text] [PDF]

				M. KAIZUKA, H. YAMABE, H. OSAWA, K. OKUMURA, and N. FUJIMOTO Thrombin Stimulates Synthesis of Type IV Collagen and Tissue Inhibitor of Metalloproteinases-1 by Cultured Human Mesangial Cells J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1516 - 1523. [Abstract] [Full Text]

				T. Miralem and D. M. Templeton Inactivation of kinase cascades in mesangial cells grown on collagen type I Am J Physiol Renal Physiol, October 1, 1998; 275(4): F585 - F594. [Abstract] [Full Text] [PDF]

				M. G. Mohaupt, T. Fischer, J. Schwobel, R. B. Sterzel, and E. Schulze-Lohoff Activation of purinergic P2Y2 receptors inhibits inducible NO synthase in cultured rat mesangial cells Am J Physiol Renal Physiol, July 1, 1998; 275(1): F103 - F110. [Abstract] [Full Text] [PDF]

				J. Turck, A. S. Pollock, L. K. Lee, H.-P. Marti, and D. H. Lovett Matrix Metalloproteinase 2(Gelatinase A) Regulates Glomerular Mesangial Cell Proliferation and Differentiation J. Biol. Chem., June 21, 1996; 271(25): 15074 - 15083. [Abstract] [Full Text] [PDF]

				H Grenz, S Carbonetto, and S. Goodman Alpha 3 beta 1 integrin is moved into focal contacts in kidney mesangial cells J. Cell Sci., January 7, 1993; 105(3): 739 - 751. [Abstract] [PDF]