This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Whittaker, P. Articles by Kloner, R. A. Search for Related Content PubMed PubMed Citation Articles by Whittaker, P. Articles by Kloner, R. A.

American Journal of Pathology, Vol 134, 879-893, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Analysis of healing after myocardial infarction using polarized light microscopy

P Whittaker, DR Boughner and RA Kloner
Cardiology Division, Harper Hospital, Wayne State University, Detroit, Michigan.

To better understand the healing process after permanent coronary artery occlusion in a canine model, the authors used polarized light microscopy. At 6 weeks after occlusion the scar collagen was mainly type I. Some regions of the scar contained a fiber lattice which appeared to be type III collagen. Collagen orientation was measured using a universal stage; subepicardial collagen was obliquely aligned (- 14.0 +/- 3.5 degrees), midmyocardial collagen circumferentially aligned (1.4 +/- 0.4 degrees) and subendocardial collagen obliquely aligned (12.7 +/- 2.1 degrees). The molecular organization of scar collagen increased from 1 to 6 weeks after occlusion. Muscle cell disarray, similar to that in hypertrophic cardiomyopathy, was seen in the viable muscle adjacent to the scar. Such abnormal organization extended as far as 1 cm from the edge of the scar. The ability of polarized light microscopy to assess these different parameters from histologic sections demonstrates that it is a useful adjunct to other methods commonly used to study myocardial healing.

This article has been cited by other articles:

				T. Yano, T. Miura, P. Whittaker, T. Miki, J. Sakamoto, Y. Nakamura, Y. Ichikawa, Y. Ikeda, H. Kobayashi, K. Ohori, et al. Macrophage Colony-Stimulating Factor Treatment After Myocardial Infarction Attenuates Left Ventricular Dysfunction by Accelerating Infarct Repair J. Am. Coll. Cardiol., February 7, 2006; 47(3): 626 - 634. [Abstract] [Full Text] [PDF]

				H. Peng, O. A. Carretero, N. Vuljaj, T.-D. Liao, A. Motivala, E. L. Peterson, and N.-E. Rhaleb Angiotensin-Converting Enzyme Inhibitors: A New Mechanism of Action Circulation, October 18, 2005; 112(16): 2436 - 2445. [Abstract] [Full Text] [PDF]

				P. Whittaker, J. Muller-Ehmsen, J. S. Dow, L. H. Kedes, and R. A. Kloner Development of abnormal tissue architecture in transplanted neonatal rat myocytes Ann. Thorac. Surg., May 1, 2003; 75(5): 1450 - 1456. [Abstract] [Full Text] [PDF]

				C. Basso, G. Thiene, M. Della Barbera, A. Angelini, M. Kirchengast, and S. Iliceto Endothelin A-receptor antagonist administration immediately after experimental myocardial infarction with reperfusion does not affect scar healing in dogs Cardiovasc Res, July 1, 2002; 55(1): 113 - 121. [Abstract] [Full Text] [PDF]

				S. D. Zimmerman, W. J. Karlon, J. W. Holmes, J. H. Omens, and J. W. Covell Structural and mechanical factors influencing infarct scar collagen organization Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H194 - H200. [Abstract] [Full Text] [PDF]

				P. Whittaker Transmyocardial revascularization: the fate of myocardial channels Ann. Thorac. Surg., December 1, 1999; 68(6): 2376 - 2382. [Abstract] [Full Text] [PDF]

				S. T. Kelley, R. Malekan, J. H. Gorman III, B. M. Jackson, R. C. Gorman, Y. Suzuki, T. Plappert, D. K. Bogen, M. G. St. J. Sutton, and L. H. Edmunds Jr Restraining Infarct Expansion Preserves Left Ventricular Geometry and Function After Acute Anteroapical Infarction Circulation, January 12, 1999; 99(1): 135 - 142. [Abstract] [Full Text] [PDF]

				T. Murakami, S. Kusachi, M. Murakami, I. Sano, T. Uesugi, M. Murakami, R. Hirami, A. Kajiyama, J. Kondo, and T. Tsuji Time-dependent changes of serum carboxy-terminal peptide of type I procollagen and carboxy-terminal telopeptide of type I collagen concentrations in patients with acute myocardial infarction after successful reperfusion: correlation with left ventricular volume indices Clin. Chem., December 1, 1998; 44(12): 2453 - 2461. [Abstract] [Full Text] [PDF]

				E. W. Hsu, A. L. Muzikant, S. A. Matulevicius, R. C. Penland, and C. S. Henriquez Magnetic resonance myocardial fiber-orientation mapping with direct histological correlation Am J Physiol Heart Circ Physiol, May 1, 1998; 274(5): H1627 - H1634. [Abstract] [Full Text] [PDF]

				C. E. Ruiz, H. P. Zhang, A. I. Butt, and P. Whittaker Percutaneous Treatment of Abdominal Aortic Aneurysm in a Swine Model : Understanding the Behavior of Aortic Aneurysm Closure Through a Serial Histopathological Analysis Circulation, October 7, 1997; 96(7): 2438 - 2448. [Abstract] [Full Text]

				B. I. Jugdutt, M. J. Joljart, and M. I. Khan Rate of Collagen Deposition During Healing and Ventricular Remodeling After Myocardial Infarction in Rat and Dog Models Circulation, July 1, 1996; 94(1): 94 - 101. [Abstract] [Full Text]

				P. Whittaker, K. Rakusan, and R. A. Kloner Transmural Channels Can Protect Ischemic Tissue : Assessment of Long-term Myocardial Response to Laser- and Needle-MadeChannels Circulation, January 1, 1996; 93(1): 143 - 152. [Abstract] [Full Text]

				P. A. Dowdy, A. Miniaci, S. P. Arnoczky, P. J. Fowler, and D. R. Boughner The Effect of Cast Immobilization on Meniscal Healing: An Experimental Study in the Dog Am. J. Sports Med., December 1, 1995; 23(6): 721 - 728. [Abstract] [PDF]

				V. Richard, C. E. Murry, and K. A. Reimer Healing of Myocardial Infarcts in Dogs : Effects of Late Reperfusion Circulation, October 1, 1995; 92(7): 1891 - 1901. [Abstract] [Full Text]