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American Journal of Pathology, Vol 134, 1039-1046, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Morphologic demonstration of adrenergic influences on the glomerulus

V Kon and MJ Karnovsky
Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2584.

Previous micropuncture studies found that increasing the adrenergic nerve activity to the kidneys elevates the pre- and postglomerular arteriolar resistances and decreases the glomerular capillary ultrafiltration coefficient (product of the filtration surface area and the hydraulic conductivity to water). To define the morphologic expression of this adrenergic effect on the glomerular capillaries the authors compared the microscopic vascular casts of entire glomeruli from right and left kidneys that were simultaneously perfusion-fixed during selective stimulation of only the left renal nerves. The maximum cross-sectional diameter of ten randomly chosen glomeruli from each stimulated and contralateral kidneys of eight rats averaged 123.7 +/- 4.1 mu in stimulated kidneys compared with a maximum diameter of 136.3 +/- 6.4 in the contralateral kidneys (P less than 0.001). The average perpendicular diameter of 100.4 +/- 1.5 mu in the stimulated kidneys was also significantly smaller than the average diameter of 110.7 +/- 1.9 mu in the contralateral kidneys (P less than 0.005). To examine if morphologic changes analogous to those found in whole glomeruli can be demonstrated at the single cell level, the authors assessed the size of mesangial cells in vitro before, during, and after exposure to the adrenergic neurotransmitter, norepinephrine. First passage mesangial cells approximately 4 weeks after explantation were studied by phase- contrast microscopy and recorded on time-lapse video recorder. The planar surface area of individual mesangial cells was measured by electronic planimeter from photographs of the video images. In response to norepinephrine (1 microM), the surface area decreased significantly on average, from 3.58 +/- 0.28 X 10(-6) sq mm to 3.38 +/- 0.27 (P less than 0.005). Washout of norepinephrine and replacement with hormone- free media in other cells led an increase in the surface area (from 2.47 +/- 0.43 X 10(-6) sq mm to 2.61 +/- 0.40, P less than 0.005). No changes were observed in cells initially bathed in hormone-free media. Thus, the morphologic equivalent of the adrenergic nerve-induced reduction in the ultrafiltration coefficient is a contraction of the glomerular corpuscle. By regulating the configuration of mesangial cells that anchor the glomerular capillary network to the vascular pole, the adrenergic nerve may concurrently determine the number of capillary channels available for filtration as well as the glomerular corpuscular volume.

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