| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 134, 1227-1232, Copyright © 1989 by American Society for Investigative Pathology
REGULAR ARTICLES |
G Sahagun, SA Moore, Z Fabry, RL Schelper and MN Hart
Department of Pathology, University of Iowa, Iowa City 52242.
Griffonia simplicifolia agglutinin (GSA) is a valuable histochemical tool in the identification of endothelium. In this study GSA labeled with fluorescein isothiocyanate (GSA-FITC) was used to purify cultures of murine cerebral microvascular endothelium. Cultures were stained with GSA-FITC, then sorted using a fluorescence-activated cell sorter (FACS). GSA-positive endothelial cells were collected, re-cultured, and subsequently re-analyzed by FACS using GSA-FITC. Cultures that initially contained 80 +/- 3 to 89 +/- 3% (X +/- SE) GSA-positive cells were purified to 98 +/- 1% positivity. Immunohistochemistry with an anti-muscle-action antibody confirmed that FACS sorting of GSA-FITC- stained cells effectively removed contaminating smooth muscle cells from endothelial cell cultures. Viability, proliferation, and prostaglandin production of the cells was unaltered by lectin staining and FACS sorting. Thus, GSA-FITC can be used in conjunction with flow cytometry to enhance the purity of murine endothelial cell cultures without adversely affecting cell viability, growth, or metabolism.
This article has been cited by other articles:
 |
|
 |
|
  J. R. Basile, K. Holmbeck, T. H. Bugge, and J. S. Gutkind MT1-MMP Controls Tumor-induced Angiogenesis through the Release of Semaphorin 4D J. Biol. Chem., March 2, 2007; 282(9): 6899 - 6905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Lister and M. J. Hickey Immune complexes alter cerebral microvessel permeability: roles of complement and leukocyte adhesion Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H694 - H704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Teng, H. R. Ansari, P. J. Oldenburg, J. Schnermann, and S. J. Mustafa Isolation and characterization of coronary endothelial and smooth muscle cells from A1 adenosine receptor-knockout mice Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1713 - H1720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. van den Heuvel, J. Horrocks, S. Bashar, S. Taylor, S. Burke, K. Hatta, J. E. Lewis, and B. A. Croy Menstrual Cycle Hormones Induce Changes in Functional Interactions between Lymphocytes and Decidual Vascular Endothelial Cells J. Clin. Endocrinol. Metab., May 1, 2005; 90(5): 2835 - 2842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E Stern and W. Zhang Cellular sources, targets and actions of constitutive nitric oxide in the magnocellular neurosecretory system of the rat J. Physiol., February 1, 2005; 562(3): 725 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Davis, J.P. M. Motion, D. A. Narmoneva, T. Takahashi, D. Hakuno, R. D. Kamm, S. Zhang, and R. T. Lee Injectable Self-Assembling Peptide Nanofibers Create Intramyocardial Microenvironments for Endothelial Cells Circulation, February 1, 2005; 111(4): 442 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Biancone, V. Cantaluppi, L. Del Sorbo, S. Russo, L. W. Tjoelker, and G. Camussi Platelet-activating Factor Inactivation by Local Expression of Platelet-activating Factor Acetyl-Hydrolase Modifies Tumor Vascularization and Growth Clin. Cancer Res., September 15, 2003; 9(11): 4214 - 4220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Simper, P. G. Stalboerger, C. J. Panetta, S. Wang, and N. M. Caplice Smooth Muscle Progenitor Cells in Human Blood Circulation, September 3, 2002; 106(10): 1199 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Ritter, M. I. Dorrell, J. Edmonds, S. F. Friedlander, and M. Friedlander From the Cover: Insulin-like growth factor 2 and potential regulators of hemangioma growth and involution identified by large-scale expression analysis PNAS, May 28, 2002; 99(11): 7455 - 7460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Bussolati, L. Biancone, P. Cassoni, S. Russo, M. Rola-Pleszczynski, G. Montrucchio, and G. Camussi PAF Produced by Human Breast Cancer Cells Promotes Migration and Proliferation of Tumor Cells and Neo-Angiogenesis Am. J. Pathol., November 1, 2000; 157(5): 1713 - 1725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Seo, N. Okamoto, M. A. Vinores, S. A. Vinores, S. F. Hackett, H. Yamada, E. Yamada, N. L. Derevjanik, W. LaRochelle, D. J. Zack, et al. Photoreceptor-Specific Expression of Platelet-Derived Growth Factor-B Results in Traction Retinal Detachment Am. J. Pathol., September 1, 2000; 157(3): 995 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Biancone, V. Cantaluppi, M. Boccellino, L. Del Sorbo, S. Russo, A. Albini, I. Stamenkovic, and G. Camussi Activation of CD40 Favors the Growth and Vascularization of Kaposi's Sarcoma J. Immunol., December 1, 1999; 163(11): 6201 - 6208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Hoerstrup, G. Zund, A. Schoeberlein, Q. Ye, P. R. Vogt, and M. I. Turina Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve Ann. Thorac. Surg., November 1, 1998; 66(5): 1653 - 1657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. G. Dong, S. Bernasconi, S. Lostaglio, R. Wainstok De Calmanovici, I. Martin-Padura, F. Breviario, C. Garlanda, S. Ramponi, A. Mantovani, and A. Vecchi A General Strategy for Isolation of Endothelial Cells From Murine Tissues : Characterization of Two Endothelial Cell Lines From the Murine Lung and Subcutaneous Sponge Implants Arterioscler. Thromb. Vasc. Biol., August 1, 1997; 17(8): 1599 - 1604. [Abstract] [Full Text]
|
|
|
|
|
|
C. Garlanda and E. Dejana Heterogeneity of Endothelial Cells : Specific Markers Arterioscler. Thromb. Vasc. Biol., July 1, 1997; 17(7): 1193 - 1202. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
