help button home button Am J Pathol ASIP WHAT IS IT?
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Juhlin, C.
Right arrow Articles by Akerstrom, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Juhlin, C.
Right arrow Articles by Akerstrom, G.

American Journal of Pathology, Vol 135, 321-328, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Parathyroid histology and cytology with monoclonal antibodies recognizing a calcium sensor of parathyroid cells

C Juhlin, J Rastad, L Klareskog, L Grimelius and G Akerstrom
Department of Surgery, University of Uppsala, Sweden.

Two monoclonal antibodies reacting with surface structures on exclusively parathyroid cells and proximal kidney tubule cells were evaluated in the microscopic diagnosis of parathyroid disease. Immunostained cryosections of adenomatous and hyperplastic parathyroid glands from 30 hypercalcemic patients with primary or uremic hyperparathroidism (HPT) showed a reduced and heterogeneous staining with the antibodies. This contrasted with the intense and even staining of normal and suppressed human parathyroid glands, as well as lymph node metastases of parathyroid carcinoma. Fine-needle aspiration biopsies and imprints of the parathyroid glands showed a conspicuous antibody staining that enabled the identification of parathyroid cells. The antibodies seem to provide new means for histologic and cytologic identification of normal and pathologic parathyroid tissue, in conjunction with their highly restricted tissue reactivity. Because one of the antibodies interacts with a surface receptor mechanism of the parathyroid cells that is involved in the sensing and gating of calcium and, thereby, also with the regulation of parathyroid hormone release, the unequivocal demonstration of reduced staining of the adenomatous and hyperplastic parathyroid tissue suggests that HPT is associated with reduced expression of the calcium sensoring mechanism of the pathologic parathyroid tissue.


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
T. B. DRÜEKE
Cell Biology of Parathyroid Gland Hyperplasia in Chronic Renal Failure
J. Am. Soc. Nephrol., June 1, 2000; 11(6): 1141 - 1152.
[Full Text]

Home page
 J. Histochem. Cytochem.Home page
S. Lundgren, T. Carling, G. Hjalm, C. Juhlin, J. Rastad, U. Pihlgren, L. Rask, G. Akerstrom, and P. Hellman
Tissue Distribution of Human gp330/Megalin, a Putative Ca2+-sensing Protein
J. Histochem. Cytochem., March 1, 1997; 45(3): 383 - 392.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by the American Society for Investigative Pathology.