This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grondahl-Hansen, J. Articles by Dano, K. Search for Related Content PubMed PubMed Citation Articles by Grondahl-Hansen, J. Articles by Dano, K.

American Journal of Pathology, Vol 135, 631-636, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Urokinase-type plasminogen activator in endothelial cells during acute inflammation of the appendix

J Grondahl-Hansen, LT Kirkeby, E Ralfkiaer, P Kristensen, LR Lund and K Dano
Department of Biochemistry and Nutrition Technical University of Denmark, Lyngby.

Urokinase and tissue-type plasminogen activators (u-PA and t-PA) were identified immunohistochemically in normal and inflamed human appendices by means of polyclonal and monoclonal antibodies. In addition, extracts of the tissues were analyzed for u-PA and t-PA by ELISA. Twelve appendices (five normal and seven with acute inflammation) were analyzed. In the normal appendices, there was a strong staining of the endothelial cells for t-PA, whereas there was negative staining for u-PA. In contrast, the endothelial cells in the inflamed appendices showed u-PA immunoreactivity, and negative or very weak reactions for t-PA. In the inflamed appendix, there was also a labeling of u-PA in fibroblast-like cells and in interstitial areas. The specificity of the staining was supported by a variety of staining controls and also by analysis of tissue extracts with ELISA, showing that on the average the inflamed appendices contained more than twice as much mu-PA per mg of protein as the normal appendices and less than one third of the amount of t-PA.

This article has been cited by other articles:

				P. Koolwijk, K. Kapiteijn, B. Molenaar, E. van Spronsen, B. van der Vecht, F. M. Helmerhorst, and V. W. M. van Hinsbergh Enhanced Angiogenic Capacity and Urokinase-Type Plasminogen Activator Expression by Endothelial Cells Isolated from Human Endometrium J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3359 - 3367. [Abstract] [Full Text] [PDF]

				H. Oka, K. Kugiyama, H. Doi, T. Matsumura, H. Shibata, L. A. Miles, S. Sugiyama, and H. Yasue Lysophosphatidylcholine Induces Urokinase-Type Plasminogen Activator and Its Receptor in Human Macrophages Partly Through Redox-Sensitive Pathway Arterioscler. Thromb. Vasc. Biol., January 1, 2000; 20(1): 244 - 250. [Abstract] [Full Text] [PDF]

				T. Plesner, N. Behrendt, and M. Ploug Structure, Function and Expression on Blood and Bone Marrow Cells of the Urokinase-Type Plasminogen Activator Receptor, uPAR Stem Cells, November 1, 1997; 15(6): 398 - 408. [Abstract] [Full Text]

				P.N. Raghunath, J. E. Tomaszewski, S. T. Brady, R. J. Caron, S. S. Okada, and E. S. Barnathan Plasminogen Activator System in Human Coronary Atherosclerosis Arterioscler. Thromb. Vasc. Biol., September 1, 1995; 15(9): 1432 - 1443. [Abstract] [Full Text]