| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 135, 947-959, Copyright © 1989 by American Society for Investigative Pathology
REGULAR ARTICLES |
PF Weller, SJ Ackerman, A Nicholson-Weller and AM Dvorak
Department of Medicine, Harvard Medical School, Boston, Massachusetts.
The morphology and function of cytoplasmic lipid bodies in human neutrophils were evaluated. By transmission electron microscopy, neutrophil lipid bodies were cytoplasmic inclusions, usually several microns in diameter, that occasionally coalesced to attain a diameter up to 7 microM. Neutrophil lipid bodies were not enveloped by membrane but were often surrounded by a more electron-dense shell at their periphery. Normal peripheral blood neutrophils contained an average of approximately one lipid body per cell. Lipid bodies appeared in greater numbers in neutrophils from inflammatory lesions. Perturbation of neutrophils during conventional methods of cell isolation and purification modestly increased lipid body numbers in neutrophils, whereas incubation of neutrophils with 1 microM oleic acid rapidly induced lipid body formation over 30 to 60 minutes. After granulocytes were incubated for 2 hours with 3H-fatty acids, including arachidonic, oleic, and palmitic acids, electron microscopic autoradiography demonstrated that lipid bodies represented the predominant intracellular sites of localization of each of the three 3H-fatty acids. There was lesser labeling noted in the perinuclear cisterna, but not in cell membranes. Virtually all of each of the three 3H-fatty acids incorporated by the neutrophils were esterified into chromatographically resolved classes of neutral lipids or phospholipids. These findings indicate that cytoplasmic lipid bodies are more prominent in neutrophils in vivo engaged in inflammatory responses and that these organelles in human neutrophils function as sites of deposition of esterified, incorporated fatty acids.
This article has been cited by other articles:
 |
|
 |
|
  H.-C. Wan, R. C. N. Melo, Z. Jin, A. M. Dvorak, and P. F. Weller Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies FASEB J, January 1, 2007; 21(1): 167 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Urasaki, J. Takasaki, T. Nagasawa, and H. Ninomiya Pivotal role of 5-lipoxygenase in the activation of human eosinophils: platelet-activating factor and interleukin-5 induce CD69 on eosinophils through the 5-lipoxygenase pathway J. Leukoc. Biol., January 1, 2001; 69(1): 105 - 112. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. M. Scarfo, P. F. Weller, and H. W. Farber Induction of endothelial cell cytoplasmic lipid bodies during hypoxia Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H294 - H301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Johnson, B. Vaughn, M. Triggiani, D. D. Swan, A. N. Fonteh, and F. H. Chilton Role of Arachidonyl Triglycerides within Lipid Bodies in Eicosanoid Formation by Human Polymorphonuclear Cells Am. J. Respir. Cell Mol. Biol., August 1, 1999; 21(2): 253 - 258. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. A. Giembycz and M. A. Lindsay Pharmacology of the Eosinophil Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Bartemes, S. McKinney, G. J. Gleich, and H. Kita Endogenous Platelet-Activating Factor Is Critically Involved in Effector Functions of Eosinophils Stimulated with IL-5 or IgG J. Immunol., March 1, 1999; 162(5): 2982 - 2989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Bozza, W. Yu, J. F. Penrose, E. S. Morgan, A. M. Dvorak, and P. F. Weller Eosinophil Lipid Bodies: Specific, Inducible Intracellular Sites for Enhanced Eicosanoid Formation J. Exp. Med., September 15, 1997; 186(6): 909 - 920. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
