This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van der Kraan, P. M. Articles by van den Berg, W. B. Search for Related Content PubMed PubMed Citation Articles by van der Kraan, P. M. Articles by van den Berg, W. B.

American Journal of Pathology, Vol 135, 1001-1014, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Development of osteoarthritic lesions in mice by "metabolic" and "mechanical" alterations in the knee joints

PM van der Kraan, EL Vitters, LB van de Putte and WB van den Berg
Department of Rheumatology, Academic Hospital St. Radboud, Nijmegen, The Netherlands.

Male, 10-week-old C57B1 10 mice received a single intraarticular injection in the knee joints with papain, iodoacetate, or collagenase. This led to osteoarthritic lesions, such as matrix depletion, chondrocyte proliferation, and osteophyte formation, in the injected knee joints within several weeks. After injection of iodoacetate and papain, the main osteoarthritic alterations were localized in the femoropatellar joint, whereas injection of collagenase led to marked osteoarthritic lesions in the femorotibial joint. The mechanism of induction of these alterations appears to differ for iodoacetate and papain on one site and collagenase on the other site. Data are presented that collagenase injection, by way of damaging ligaments and tendons, destabilizes the knee joint eventually leading to osteoarthritic alterations. In contrast, injection of papain or iodoacetate directly interferes with cartilage metabolism resulting in osteoarthritic changes.

This article has been cited by other articles:

				C Boileau, F Poirier, J-P Pelletier, M Guevremont, N Duval, J Martel-Pelletier, and P Reboul Intracellular localisation of galectin-3 has a protective role in chondrocyte survival Ann Rheum Dis, February 1, 2008; 67(2): 175 - 181. [Abstract] [Full Text] [PDF]

				E N Blaney Davidson, E L Vitters, P M van der Kraan, and W B van den Berg Expression of transforming growth factor-{beta} (TGF{beta}) and the TGF{beta} signalling molecule SMAD-2P in spontaneous and instability-induced osteoarthritis: role in cartilage degradation, chondrogenesis and osteophyte formation Ann Rheum Dis, November 1, 2006; 65(11): 1414 - 1421. [Abstract] [Full Text] [PDF]

				R. E. Guzman, M. G. Evans, S. Bove, B. Morenko, and K. Kilgore Mono-Iodoacetate-Induced Histologic Changes in Subchondral Bone and Articular Cartilage of Rat Femorotibial Joints: An Animal Model of Osteoarthritis Toxicol Pathol, October 1, 2003; 31(6): 619 - 624. [Abstract] [PDF]

				A. Scharstuhl, H. L. Glansbeek, H. M. van Beuningen, E. L. Vitters, P. M. van der Kraan, and W. B. van den Berg Inhibition of Endogenous TGF-{beta} During Experimental Osteoarthritis Prevents Osteophyte Formation and Impairs Cartilage Repair J. Immunol., July 1, 2002; 169(1): 507 - 514. [Abstract] [Full Text] [PDF]