This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hopman, A. H. Articles by Ramaekers, F. C. Search for Related Content PubMed PubMed Citation Articles by Hopman, A. H. Articles by Ramaekers, F. C.

American Journal of Pathology, Vol 135, 1105-1117, Copyright © 1989 by American Society for Investigative Pathology

REGULAR ARTICLES

Detection of numerical chromosome aberrations in bladder cancer by in situ hybridization

AH Hopman, PJ Poddighe, AW Smeets, O Moesker, JL Beck, GP Vooijs and FC Ramaekers
Department of Pathology, University Hospital Nijmegen, The Netherlands.

The nuclear DNA content of 53 transitional cell carcinomas (TCCs) of the urinary bladder, as determined by flow cytometry (FCM), was compared with chromosome ploidy as detected by nonradioactive in situ hybridization (ISH). For this purpose, probes for repetitive DNA targets in the (peri) centromeric region of chromosomes 1 and 18 were used. Hybridization results with both probes of 35 TCCs, which had a DNA index of approximately 1.0 as concluded from FCM, showed evident chromosome 1 aberrations in approximately 25% of the tumors, and in a few cases an aberration for chromosome 18 was detected. Comparison of the ISH spot numbers for both chromosomes showed in most cases a higher number for chromosome 1 than for chromosome 18. ISH on 18 cases of TCCs, which showed a single peak in FCM with a DNA-index of 1.2 to 3.2, exhibited a profound heterogeneity. In these TCCs the ratio between chromosomes 1 and 18 varied over a wide range, resulting in cases showing more hybridization signals for chromosome 1 than for chromosome 18 or the opposite. Furthermore, using ISH minor cell populations showing polyploidization and giant cells containing numerous ISH signals could occasionally be detected. Results showed that interphase cytogenetics by ISH enable a fast screening of numerical chromosome aberrations and detection of different cell populations within one tumor, which was apparently homogeneous according to FCM.

This article has been cited by other articles:

				L. Buonamici, M. Serra, L. Losi, and V. Eusebi Application of CARD-ISH for Assessment of Numerical Chromosome Aberrations in Interphase Nuclei of Human Tumor Cells International Journal of Surgical Pathology, July 1, 2000; 8(3): 201 - 206. [Abstract] [PDF]

				J. M. Davison, T. W. Morgan, B.-L. Hsi, S. Xiao, and J. A. Fletcher Subtracted, Unique-Sequence, In Situ Hybridization : Experimental and Diagnostic Applications Am. J. Pathol., November 1, 1998; 153(5): 1401 - 1409. [Abstract] [Full Text] [PDF]

				M. Oya, T. Yao, and M. Tsuneyoshi Numerical Chromosomal Aberration in NodePositive Early Gastric Carcinomas: Analysis by Fluorescence in Situ Hybridization and Immunohistochemical Staining International Journal of Surgical Pathology, October 1, 1998; 6(4): 189 - 196. [Abstract] [PDF]