This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ko, L. W. Articles by Blass, J. P. Search for Related Content PubMed PubMed Citation Articles by Ko, L. W. Articles by Blass, J. P.

American Journal of Pathology, Vol 136, 867-879, Copyright © 1990 by American Society for Investigative Pathology

REGULAR ARTICLES

Expression in cultured human neuroblastoma cells of epitopes associated with affected neurons in Alzheimer's disease

LW Ko, KF Sheu, O Young, H Thaler and JP Blass
Altschul Laboratory for Dementia Research, Burke Rehabilitation Center, Cornell University Medical College, White Plains, New York 10605.

Of three human neuroblastoma lines tested, IMR32K (and IMR32 parental line) was the only cell line that, after its exposure to a differentiation medium, consistently developed materials recognized immunocytochemically by a panel of antibodies against paired helical filaments (PHF). Ultrastructurally, these cells accumulated, at their perikarya and neuritic extensions, spatially discrete arrays of fibrils, which occasionally occurred in twisted pairs. When these fibrillar structures appeared as paired helices, they exhibited dimensions and configurations reminiscent of PHF found in affected Alzheimer neurons, although less compact. Immunoelectron microscope examinations of the fibrillar structures in these neuroblastoma cells with one of these anti-PHF immunoprobes revealed that only subsets of fibrillar structures that appeared thickened or aggregated to form bundles were selectively immunolabeled. Cultures of these immortal neuroblastoma lines may provide a convenient model for studying aspects of PHF formation that are hard to examine in Alzheimer brain obtained at autopsy.

This article has been cited by other articles:

				T. Ariga, W. D. Jarvis, and R. K. Yu Role of sphingolipid-mediated cell death in neurodegenerative diseases J. Lipid Res., January 1, 1998; 39(1): 1 - 16. [Abstract] [Full Text]

				R. J. Mark, Z. Pang, J. W. Geddes, K. Uchida, and M. P. Mattson Amyloid beta -Peptide Impairs Glucose Transport in Hippocampal and Cortical Neurons: Involvement of Membrane Lipid Peroxidation J. Neurosci., February 1, 1997; 17(3): 1046 - 1054. [Abstract] [Full Text] [PDF]

				I. Kill, R. Faragher, K Lawrence, and S Shall The expression of proliferation-dependent antigens during the lifespan of normal and progeroid human fibroblasts in culture J. Cell Sci., January 2, 1994; 107(2): 571 - 579. [Abstract] [PDF]