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American Journal of Pathology, Vol 136, 1021-1030, Copyright © 1990 by American Society for Investigative Pathology

REGULAR ARTICLES

In vivo expression of perforin by natural killer cells during a viral infection. Studies on uveitis produced by herpes simplex virus type I

LH Young, CS Foster and JD Young
Retina Service, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston.

A potent cytolytic pore-forming protein (PFP, perforin, or cytolysin) is associated with the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. The role of PFP/perforin in cytolytic reactions carried out in vivo is still unclear. Here, the authors performed immunohistochemical analysis using antibodies monospecific for perforin and made use of a murine uveitis model produced by intracameral inoculation of herpes simplex virus I (HSV-I). The main cell infiltrate found in the anterior segment of virus- inoculated eyes consisted of Thy-1+/asialo GM1+/CD8-/CD4- cells, presumably representing NK cells. Perforin staining was detected mainly in cells bearing this phenotype. Perforin was only detected in cells displaying the large granular lymphocyte morphology. A small number of perforin-positive cells (less than 5%) colabeled as CD8+, indicating that these cells could have belonged to the CTL lineage. These observations show for the first time the presence of perforin- containing NK cells in tissues of animals undergoing acute viral infections.


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M. Tanigawa, J. E. Bigger, M. Y. Kanter, and S. S. Atherton
Natural Killer Cells Prevent Direct Anterior-to-Posterior Spread of Herpes Simplex Virus Type 1 in the Eye
Invest. Ophthalmol. Vis. Sci., January 1, 2000; 41(1): 132 - 137.
[Abstract] [Full Text]


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Copyright © 1990 by the American Society for Investigative Pathology.