| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 136, 1235-1246, Copyright © 1990 by American Society for Investigative Pathology
REGULAR ARTICLES |
DG Greenhalgh, KH Sprugel, MJ Murray and R Ross
Department of Surgery, University of Washington, Seattle.
To examine the effects of recombinant growth factors in vivo, impaired wound healing was studied in genetically diabetic C57BL/KsJ-db/db mice. Large full-thickness skin wounds made on the backs of these mice exhibited significant delays in the entry of inflammatory cells into the wound, the formation of granulation tissue, and in wound closure when compared to their nondiabetic littermates. Recombinant human platelet-derived growth factor (rPDGF-BB, 1 or 10 micrograms), recombinant human basic fibroblast growth factor (rbFGF, 1 micrograms), or combinations of both were applied topically to the wounds for 5 to 14 days after wounding. Diabetic mouse wounds treated with rPDGF-BB or rbFGF had many more fibroblasts and capillaries in the wound bed at 10 and 21 days than did wounds treated with the vehicle alone. The animals treated with growth factors also had significantly greater wound closure at 21 days than those treated with the vehicle. Combinations of rPDGF-BB and rbFGF improved all parameters of healing but not to a greater extent than either growth factor alone. The effectiveness of rPDGF-BB and rbFGF suggest that recombinant growth factors may be useful in the treatment of patients with deficient wound repair.
This article has been cited by other articles:
 |
|
 |
|
  K. Maruyama, J. Asai, M. Ii, T. Thorne, D. W. Losordo, and P. A. D'Amore Decreased Macrophage Number and Activation Lead to Reduced Lymphatic Vessel Formation and Contribute to Impaired Diabetic Wound Healing Am. J. Pathol., April 1, 2007; 170(4): 1178 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Matei, M. Satpathy, L. Cao, Y.-C. Lai, H. Nakshatri, and D. B. Donner The Platelet-derived Growth Factor Receptor {alpha} Is Destabilized by Geldanamycins in Cancer Cells J. Biol. Chem., January 5, 2007; 282(1): 445 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Asai, H. Takenaka, K. F. Kusano, M. Ii, C. Luedemann, C. Curry, E. Eaton, A. Iwakura, Y. Tsutsumi, H. Hamada, et al. Topical Sonic Hedgehog Gene Therapy Accelerates Wound Healing in Diabetes by Enhancing Endothelial Progenitor Cell-Mediated Microvascular Remodeling Circulation, May 23, 2006; 113(20): 2413 - 2424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Schatteman and N. Ma Old Bone Marrow Cells Inhibit Skin Wound Vascularization Stem Cells, March 1, 2006; 24(3): 717 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Krampert, S. Kuenzle, S. N.-M. Thai, N. Lee, M. L. Iruela-Arispe, and S. Werner ADAMTS1 Proteinase Is Up-regulated in Wounded Skin and Regulates Migration of Fibroblasts and Endothelial Cells J. Biol. Chem., June 24, 2005; 280(25): 23844 - 23852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Mace, S. L. Hansen, C. Myers, D. M. Young, and N. Boudreau HOXA3 induces cell migration in endothelial and epithelial cells promoting angiogenesis and wound repair J. Cell Sci., June 15, 2005; 118(12): 2567 - 2577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Puolakkainen, A. D. Bradshaw, R. A. Brekken, M. J. Reed, T. Kyriakides, S. E. Funk, M. D. Gooden, R. B. Vernon, T. N. Wight, P. Bornstein, et al. SPARC-thrombospondin-2-double-null Mice Exhibit Enhanced Cutaneous Wound Healing and Increased Fibrovascular Invasion of Subcutaneous Polyvinyl Alcohol Sponges J. Histochem. Cytochem., May 1, 2005; 53(5): 571 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lobmann, G. Schultz, and H. Lehnert Proteases and the Diabetic Foot Syndrome: Mechanisms and Therapeutic Implications Diabetes Care, February 1, 2005; 28(2): 461 - 471. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Galeano, D. Altavilla, D. Cucinotta, G. T. Russo, M. Calo, A. Bitto, H. Marini, R. Marini, E. B. Adamo, P. Seminara, et al. Recombinant Human Erythropoietin Stimulates Angiogenesis and Wound Healing in the Genetically Diabetic Mouse Diabetes, September 1, 2004; 53(9): 2509 - 2517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Theopold, F. Yao, and E. Eriksson Gene Therapy in the Treatment of Lower Extremity Wounds International Journal of Lower Extremity Wounds, June 1, 2004; 3(2): 69 - 79. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Liu, T. Desta, H. He, and D. T. Graves Diabetes Alters the Response to Bacteria by Enhancing Fibroblast Apoptosis Endocrinology, June 1, 2004; 145(6): 2997 - 3003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Hansen, C. A. Myers, A. Charboneau, D. M. Young, and N. Boudreau HoxD3 Accelerates Wound Healing in Diabetic Mice Am. J. Pathol., December 1, 2003; 163(6): 2421 - 2431. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. Stepanovic, O. Awad, C. Jiao, M. Dunnwald, and G. C. Schatteman Leprdb Diabetic Mouse Bone Marrow Cells Inhibit Skin Wound Vascularization but Promote Wound Healing Circ. Res., June 13, 2003; 92(11): 1247 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jacobi, J. J. Jang, U. Sundram, H. Dayoub, L. F. Fajardo, and J. P. Cooke Nicotine Accelerates Angiogenesis and Wound Healing in Genetically Diabetic Mice Am. J. Pathol., July 1, 2002; 161(1): 97 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Buetow, J. R. Crosby, W. E. Kaminski, R. K. Ramachandran, P. Lindahl, P. Martin, C. Betsholtz, R. A. Seifert, E. W. Raines, and D. F. Bowen-Pope Platelet-Derived Growth Factor B-Chain of Hematopoietic Origin Is Not Necessary for Granulation Tissue Formation and Its Absence Enhances Vascularization Am. J. Pathol., November 1, 2001; 159(5): 1869 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Pierce Inflammation in Nonhealing Diabetic Wounds : The Space-Time Continuum Does Matter Am. J. Pathol., August 1, 2001; 159(2): 399 - 403. [Full Text]
|
|
|
|
|
|
M. T. Goova, J. Li, T. Kislinger, W. Qu, Y. Lu, L. G. Bucciarelli, S. Nowygrod, B. M. Wolf, X. Caliste, S. F. Yan, et al. Blockade of Receptor for Advanced Glycation End-Products Restores Effective Wound Healing in Diabetic Mice Am. J. Pathol., August 1, 2001; 159(2): 513 - 525. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. D. Mulder Diabetic foot ulcers: old problems--new technologies Nephrol. Dial. Transplant., April 1, 2001; 16(4): 695 - 698. [Full Text] [PDF]
|
|
|
|
|
|
D. Altavilla, A. Saitta, D. Cucinotta, M. Galeano, B. Deodato, M. Colonna, V. Torre, G. Russo, A. Sardella, G. Urna, et al. Inhibition of Lipid Peroxidation Restores Impaired Vascular Endothelial Growth Factor Expression and Stimulates Wound Healing and Angiogenesis in the Genetically Diabetic Mouse Diabetes, March 1, 2001; 50(3): 667 - 674. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. E. Rayner, A. J. Cowin, J. G. Robertson, R. D. Cooter, R. C. Harries, G. O. Regester, G. W. Smithers, C. Goddard, and D. A. Belford Mitogenic whey extract stimulates wound repair activity in vitro and promotes healing of rat incisional wounds Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2000; 278(6): R1651 - R1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Powell, R. C. Mifflin, J. D. Valentich, S. E. Crowe, J. I. Saada, and A. B. West Myofibroblasts. I. Paracrine cells important in health and disease Am J Physiol Cell Physiol, July 1, 1999; 277(1): C1 - C19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Crosby, K. A. Tappan, R. A. Seifert, and D. F. Bowen-Pope Chimera Analysis Reveals That Fibroblasts and Endothelial Cells Require Platelet-Derived Growth Factor Receptorß Expression for Participation in Reactive Connective Tissue Formation in Adults but Not during Development Am. J. Pathol., May 1, 1999; 154(5): 1315 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Chang, D. B. Hom, R. P. Agarwal, K. Pernell, J. C. Manivel, and C. Song Effects of Basic Fibroblast Growth Factor on Irradiated Porcine Skin Flaps Arch Otolaryngol Head Neck Surg, March 1, 1998; 124(3): 307 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L Guo, L Degenstein, and E Fuchs Keratinocyte growth factor is required for hair development but not for wound healing. Genes & Dev., January 15, 1996; 10(2): 165 - 175. [Abstract] [PDF]
|
|
|
|
|
|
D. G. Gilbertson, M. E. Duff, J. W. West, J. D. Kelly, P. O. Sheppard, P. D. Hofstrand, Z. Gao, K. Shoemaker, T. R. Bukowski, M. Moore, et al. Platelet-derived Growth Factor C (PDGF-C), a Novel Growth Factor That Binds to PDGF alpha and beta Receptor J. Biol. Chem., July 13, 2001; 276(29): 27406 - 27414. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
