| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 137, 179-185, Copyright © 1990 by American Society for Investigative Pathology
REGULAR ARTICLES |
GJ Raugi, JS Mullen, DH Bark, T Okada and MR Mayberg
Medical Service, Veterans Administration Medical Center, Seattle, WA 98108.
The balloon catheter injury model was used to determine the relative contributions of vascular smooth muscle cells (SMC) and platelets to thrombospondin (TSP) antigen deposition in the artery wall. Rat carotid arteries were denuded of endothelium, exposing the thrombogenic subendothelial extracellular matrix (ECM) to the circulation. Rats were killed after 1 hour, or 5, 10, or 20 days. Thrombospondin antigen deposition in the injured arteries was assessed using a specific polyclonal antiserum raised in rabbit against rat platelet TSP and a sensitive silver-enhanced immunogold staining method. Faint immunostaining for TSP antigen was detected, associated mostly with cells, in the media of the carotid artery of the nonoperated controls. One hour after balloon catheter injury, however, prominent cell- associated immunostaining was evident in the media; extracellular matrix staining was negligible. At this time, large foci of immunostaining were present on the lumenal surface of the vessel. Intimal proliferation was evident on most stained sections of tissue taken 5 days after balloon injury. Thrombospondin antigen immunostaining was markedly increased compared to nonoperated controls in all sections, regardless of the degree of intimal thickening. Thrombospondin immunostaining remained associated with cells in the neointima and media; extracellular matrix staining remained negligible. Ten days after endothelial injury, immunostaining for TSP antigen was detected in all layers of the artery, but was greater in the neointima and media. Reaction product was still associated only with cells. Thrombospondin antigen levels, as detected by this procedure, remained high in the injured tissue through 10 days of observation but appeared less prominent 20 days after injury. At this time extracellular matrix staining was obvious and cell-associated staining was reduced. These data support the hypotheses that thrombospondin (TSP) expression by vascular smooth muscle cells is an early response to injury and that the primary source of TSP antigen in injured artery is the vascular smooth muscle cells (SMC). These results support data derived from in vitro studies of TSP secretion.
This article has been cited by other articles:
 |
|
 |
|
  J. R. Wu-Wong, M. Nakane, J. Ma, X. Ruan, and P. E. Kroeger Elevated phosphorus modulates vitamin D receptor-mediated gene expression in human vascular smooth muscle cells Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1592 - F1604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Moura, M. Tjwa, P. Vandervoort, K. Cludts, and M. F. Hoylaerts Thrombospondin-1 Activates Medial Smooth Muscle Cells and Triggers Neointima Formation Upon Mouse Carotid Artery Ligation Arterioscler. Thromb. Vasc. Biol., October 1, 2007; 27(10): 2163 - 2169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. I. Stenina, E. J. Topol, and E. F. Plow Thrombospondins, Their Polymorphisms, and Cardiovascular Disease Arterioscler. Thromb. Vasc. Biol., September 1, 2007; 27(9): 1886 - 1894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Raman, I. Krukovets, T. E. Marinic, P. Bornstein, and O. I. Stenina Glycosylation Mediates Up-regulation of a Potent Antiangiogenic and Proatherogenic Protein, Thrombospondin-1, by Glucose in Vascular Smooth Muscle Cells J. Biol. Chem., February 23, 2007; 282(8): 5704 - 5714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sezaki, S. Hirohata, A. Iwabu, K. Nakamura, K. Toeda, T. Miyoshi, H. Yamawaki, K. Demircan, S. Kusachi, Y. Shiratori, et al. Thrombospondin-1 Is Induced in Rat Myocardial Infarction and Its Induction Is Accelerated by Ischemia/Reperfusion Experimental Biology and Medicine, October 1, 2005; 230(9): 621 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. McLaughlin, M. R. Mazzoni, J. H. Cleator, L. Earls, A. L. Perdigoto, J. D. Brooks, J. A. S. Muldowney III, D. E. Vaughan, and H. E. Hamm Thrombin Modulates the Expression of a Set of Genes Including Thrombospondin-1 in Human Microvascular Endothelial Cells J. Biol. Chem., June 10, 2005; 280(23): 22172 - 22180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Willis, D. Pierre-Paul, B. E. Sumpio, and V. Gahtan Vascular Smooth Muscle Cell Migration: Current Research and Clinical Implications Vascular and Endovascular Surgery, January 1, 2004; 38(1): 11 - 23. [Abstract] [PDF]
|
|
|
|
 |
|
 L. A. Maile and D. R. Clemmons Integrin-Associated Protein Binding Domain of Thrombospondin-1 Enhances Insulin-Like Growth Factor-I Receptor Signaling in Vascular Smooth Muscle Cells Circ. Res., November 14, 2003; 93(10): 925 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. I. Stenina, I. Krukovets, K. Wang, Z. Zhou, F. Forudi, M. S. Penn, E. J. Topol, and E. F. Plow Increased Expression of Thrombospondin-1 in Vessel Wall of Diabetic Zucker Rat Circulation, July 1, 2003; 107(25): 3209 - 3215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-l. A. Hannah, T. M. Misenheimer, D. S. Annis, and D. F. Mosher A Polymorphism in Thrombospondin-1 Associated with Familial Premature Coronary Heart Disease Causes a Local Change in Conformation of the Ca2+-binding Repeats J. Biol. Chem., March 7, 2003; 278(11): 8929 - 8934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ichii, H. Koyama, S. Tanaka, A. Shioi, Y. Okuno, S. Otani, and Y. Nishizawa Thrombospondin-1 Mediates Smooth Muscle Cell Proliferation Induced by Interaction With Human Platelets Arterioscler. Thromb. Vasc. Biol., August 1, 2002; 22(8): 1286 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dufourcq, T. Couffinhal, P. Alzieu, D. Daret, C. Moreau, C. Duplaa, and J. Bonnet Vitronectin is up-regulated after vascular injury and vitronectin blockade prevents neointima formation Cardiovasc Res, March 1, 2002; 53(4): 952 - 962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chamberlain, J. Gunn, S. E. Francis, C. M. Holt, N. D. Arnold, D. C. Cumberland, M. W.J. Ferguson, and D. C. Crossman TGF{beta} is active, and correlates with activators of TGF{beta}, following porcine coronary angioplasty Cardiovasc Res, April 1, 2001; 50(1): 125 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ichii, H. Koyama, S. Tanaka, S. Kim, A. Shioi, Y. Okuno, E. W. Raines, H. Iwao, S. Otani, and Y. Nishizawa Fibrillar Collagen Specifically Regulates Human Vascular Smooth Muscle Cell Genes Involved in Cellular Responses and the Pericellular Matrix Environment Circ. Res., March 16, 2001; 88(5): 460 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-M. Zhao, Y. Hu, G. G. Miller, R. N. Mitchell, and P. Libby Association of Thrombospondin-1 and Cardiac Allograft Vasculopathy in Human Cardiac Allografts Circulation, January 30, 2001; 103(4): 525 - 531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Riessen, M. Fenchel, H. Chen, D. I. Axel, K. R. Karsch, and J. Lawler Cartilage Oligomeric Matrix Protein (Thrombospondin-5) Is Expressed by Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., January 1, 2001; 21(1): 47 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chen, K. Guo, J. Yang, W. A. Frazier, J. M. Isner, and V. Andres Vascular smooth muscle cell growth arrest on blockade of thrombospondin-1 requires p21Cip1/WAF1 Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H1100 - H1106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chen, T. Asahara, K. Krasinski, B. Witzenbichler, J. Yang, M. Magner, M. Kearney, W. A. Frazier, J. M. Isner, and V. Andres Antibody Blockade of Thrombospondin Accelerates Reendothelialization and Reduces Neointima Formation in Balloon-Injured Rat Carotid Artery Circulation, August 24, 1999; 100(8): 849 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Smith, S. R. Bryant, L. L. Couper, C. P. H. Vary, P. J. Gotwals, V. E. Koteliansky, and V. Lindner Soluble Transforming Growth Factor-ß Type II Receptor Inhibits Negative Remodeling, Fibroblast Transdifferentiation, and Intimal Lesion Formation But Not Endothelial Growth Circ. Res., May 28, 1999; 84(10): 1212 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Stouffer, Z. Hu, M. Sajid, H. Li, G. Jin, M. T. Nakada, S. R. Hanson, and M. S. Runge ß3 Integrins Are Upregulated After Vascular Injury and Modulate Thrombospondin- and Thrombin-Induced Proliferation of Cultured Smooth Muscle Cells Circulation, March 10, 1998; 97(9): 907 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hedin, J. Thyberg, J. Roy, A. Dumitrescu, and P. K. Tran Role of Tyrosine Kinases in Extracellular Matrix–Mediated Modulation of Arterial Smooth Muscle Cell Phenotype Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 1977 - 1984. [Abstract] [Full Text]
|
|
|
|
|
|
M. K. Patel, J. S. Lymn, G. F. Clunn, and A. D. Hughes Thrombospondin-1 Is a Potent Mitogen and Chemoattractant for Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2107 - 2114. [Abstract] [Full Text]
|
|
|
|
|
|
L. Liaw, D. M. Lombardi, M. M. Almeida, S. M. Schwartz, D. deBlois, and C. M. Giachelli Neutralizing Antibodies Directed Against Osteopontin Inhibit Rat Carotid Neointimal Thickening After Endothelial Denudation Arterioscler. Thromb. Vasc. Biol., January 1, 1997; 17(1): 188 - 193. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. H. Gibbons and V. J. Dzau The Emerging Concept of Vascular Remodeling N. Engl. J. Med., May 19, 1994; 330(20): 1431 - 1438. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
