help button home button Am J Pathol Epitomics Buy 2 Antibodies Get 1 Free Special Offer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schreiber, G.
Right arrow Articles by Dubeau, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schreiber, G.
Right arrow Articles by Dubeau, L.

American Journal of Pathology, Vol 137, 653-658, Copyright © 1990 by American Society for Investigative Pathology

REGULAR ARTICLES

C-myc proto-oncogene amplification detected by polymerase chain reaction in archival human ovarian carcinomas

G Schreiber and L Dubeau
Kenneth Norris Jr. Comprehensive Cancer Center, University of Southern California, Los Angeles 90033.

Polymerase chain reaction (PCR) technology was used to examine the state of amplification of the proto-oncogene c-myc in archival ovarian carcinomas. Sequences from the c-myc gene and from a control gene were amplified simultaneously by PCR and the ratios of the two products measured. The results provided an accurate measurement of the relative number of copies of the two genes in each tumor genome if the control and test sequences amplified by PCR were of equal lengths. The results were not affected by the number of PCR cycles used. This technique should facilitate gene amplification studies in clinical medicine. Increased c-myc copy number was found in 17% of the 30 cases examined when a control from the same chromosome as c-myc was used, but in 37% of cases if a control from another chromosome was used. This underlines the importance of the genetic location of the selected control genes for such studies.


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
R. Wu, L. Lin, D. G. Beer, L. H. Ellenson, B. J. Lamb, J.-M. Rouillard, R. Kuick, S. Hanash, D. R. Schwartz, E. R. Fearon, et al.
Amplification and Overexpression of the L-MYC Proto-Oncogene in Ovarian Carcinomas
Am. J. Pathol., May 1, 2003; 162(5): 1603 - 1610.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. P. Rodrigo, L. A. García, S. Ramos, P. S. Lazo, and C. Suárez
EMS1 Gene Amplification Correlates with Poor Prognosis in Squamous Cell Carcinomas of the Head and Neck
Clin. Cancer Res., August 1, 2000; 6(8): 3177 - 3182.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the American Society for Investigative Pathology.