This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rouan, S. K. Articles by Rhodes, C. T. Search for Related Content PubMed PubMed Citation Articles by Rouan, S. K. Articles by Rhodes, C. T.

American Journal of Pathology, Vol 137, 779-787, Copyright © 1990 by American Society for Investigative Pathology

REGULAR ARTICLES

Reversal of colchicine-induced mitotic arrest in Chinese hamster cells with a colchicine-specific monoclonal antibody

SK Rouan, IG Otterness, AC Cunningham, HE Holden and CT Rhodes
Department of Pharmaceutics, University of Rhode Island, Kingston.

The ability of a high-affinity colchicine-binding monoclonal antibody to reverse the effects of colchicine on Chinese hamster ovary cells was investigated. Using flow cytometry, a complete mitotic blockade was demonstrated after 16 hours with 2.5 x 10(-7) mol/l (molar) colchicine. Colchicine-induced changes were reversible when equimolar antibody was added simultaneously with or up to 6 hours after colchicine. With further delay in addition of antibody, a progressive irreversible increase in mitotic blockade and increase in mean cell size was observed. Prolonged colchicine exposure, without antibody reversal, led to polyploidy and structural chromosome breakage. Early antibody reversal restored cells to the diploid state, whereas delayed reversal resulted in a time-dependent increase in polyploidy. Colchicine-induced polyploidy and chromosomal aberrations may be the basis for both colchicine toxicity and the time-dependent increase in irreversibility of colchicine effects.

This article has been cited by other articles:

				G. Korpanty, J. G. Carbon, P. A. Grayburn, J. B. Fleming, and R. A. Brekken Monitoring Response to Anticancer Therapy by Targeting Microbubbles to Tumor Vasculature Clin. Cancer Res., January 1, 2007; 13(1): 323 - 330. [Abstract] [Full Text] [PDF]

				Y. Liang, R. A Brekken, and S. M Hyder Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the anti-proliferative activity of anti-hormones. Endocr. Relat. Cancer, September 1, 2006; 13(3): 905 - 919. [Abstract] [Full Text] [PDF]

				R. A. Brekken, J. P. Overholser, V. A. Stastny, J. Waltenberger, J. D. Minna, and P. E. Thorpe Selective Inhibition of Vascular Endothelial Growth Factor (VEGF) Receptor 2 (KDR/Flk-1) Activity by a Monoclonal Anti-VEGF Antibody Blocks Tumor Growth in Mice Cancer Res., September 1, 2000; 60(18): 5117 - 5124. [Abstract] [Full Text]

				F. J. Baud, A. Sabouraud, E. Vicaut, P. Taboulet, J. Lang, C. Bismuth, J. M. Rouzioux, and J.-M. Scherrmann Treatment of Severe Colchicine Overdose with Colchicine-Specific Fab Fragments N. Engl. J. Med., March 9, 1995; 332(10): 642 - 645. [Full Text] [PDF]