| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 137, 801-810, Copyright © 1990 by American Society for Investigative Pathology
REGULAR ARTICLES |
W Casscells, H Kimura, JA Sanchez, ZX Yu and VJ Ferrans
Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
Light microscopic immunohistochemical studies were performed to evaluate the distribution of fibronectin in paraffin sections of p- formaldehyde-fixed normal rat hearts and the hearts of rats that had undergone ligation of the left coronary artery. A peroxidase-labeled antibody technique was used, together with appropriate immunohistochemical control procedures, for the localization of fibronectin in normal hearts and in the hearts of sham-operated animals. Fibronectin was localized in the interstitial space between myocytes, and beneath arterial, venous, and capillary endothelium. At 4 hours after coronary ligation, fibronectin was localized in a patchy fashion in the cytoplasm and interstitial space of some of the myocytes in the area supplied by the ligated vessel. At 24 hours, there was more intense, homogeneous staining in necrotic myocytes in the infarcted area and in the capillary endothelium in the border zone. At 48 hours, the intensity of staining for fibronectin was maximal in and between the necrotic myocytes in the center of the infarct and in proliferating and migrating capillaries and fibroblasts in the border zone. Similar patterns of localization were observed at 3 and 7 days after coronary ligation, but with progressive decreases in the intensity of staining. Two sources of fibronectin appeared to have contributed to these changes: plasma fibronectin diffusing through damaged blood vessels would account for the early staining observed in necrotic myocytes in the center of the infarct, whereas de novo synthesis of fibronectin by connective tissue cells and endothelial cells in sprouting capillaries would be responsible for the subsequent staining observed in viable capillaries in the border zone of the infarct. Known properties of fibronectin in vitro, combined with these in vivo observations, indicate that fibronectin may influence the thrombotic, inflammatory, angiogenic, and fibrotic processes involved in infarct healing.
This article has been cited by other articles:
 |
|
 |
|
  J. I. Virag and C. E. Murry Myofibroblast and Endothelial Cell Proliferation during Murine Myocardial Infarct Repair Am. J. Pathol., December 1, 2003; 163(6): 2433 - 2440. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Tokuno, K. Hinokiyama, K. Tokuno, C. Lowbeer, L.-O. Hansson, and G. Valen Spontaneous Ischemic Events in the Brain and Heart Adapt the Hearts of Severely Atherosclerotic Mice to Ischemia Arterioscler. Thromb. Vasc. Biol., June 1, 2002; 22(6): 995 - 1001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. PALMANS, J. C. KIPS, and R. A. PAUWELS Prolonged Allergen Exposure Induces Structural Airway Changes in Sensitized Rats Am. J. Respir. Crit. Care Med., February 1, 2000; 161(2): 627 - 635. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Nawata, I. Ohno, S. Isoyama, J. Suzuki, S. Miura, J. Ikeda, and K. Shirato Differential expression of {alpha}1, {alpha}3 and {alpha}5 integrin subunits in acute and chronic stages of myocardial infarction in rats Cardiovasc Res, August 1, 1999; 43(2): 371 - 381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Murakami, S. Kusachi, M. Murakami, I. Sano, T. Uesugi, M. Murakami, R. Hirami, A. Kajiyama, J. Kondo, and T. Tsuji Time-dependent changes of serum carboxy-terminal peptide of type I procollagen and carboxy-terminal telopeptide of type I collagen concentrations in patients with acute myocardial infarction after successful reperfusion: correlation with left ventricular volume indices Clin. Chem., December 1, 1998; 44(12): 2453 - 2461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Casscells Growth Factor Therapies for Vascular Injury and Ischemia Circulation, June 1, 1995; 91(11): 2699 - 2702. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
