This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weller, P. F. Articles by Dvorak, A. M. Search for Related Content PubMed PubMed Citation Articles by Weller, P. F. Articles by Dvorak, A. M.

American Journal of Pathology, Vol 138, 141-148, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Cytoplasmic lipid bodies of human eosinophils. Subcellular isolation and analysis of arachidonate incorporation

PF Weller, RA Monahan-Earley, HF Dvorak and AM Dvorak
Department of Medicine, Harvard Medical School, Boston, Massachusetts.

Lipid bodies are non-membrane-bound cytoplasmic inclusions that are prominent in leukocytes engaged in inflammatory responses. As demonstrated by electron microscopic autoradiography, lipid bodies can serve as intracellular sites of 3H-arachidonic acid localization in eosinophils and other cells. To evaluate the role of lipid bodies as stores of esterified arachidonate, subcellular fractionation of lipid- body-rich human eosinophils was used to isolate lipid bodies free of other organelles. In lipid bodies isolated from 3H-arachidonate-labeled eosinophils, 3H-arachidonate was esterified almost totally in glycerolipids, predominantly in classes of phospholipids, including phosphatidyl-inositol and phosphatidylcholine. Lipid bodies, especially in leukocytes participating in inflammation, could represent intracellular sources of esterified arachidonate available for eicosanoid formation.

This article has been cited by other articles:

				H.-C. Wan, R. C. N. Melo, Z. Jin, A. M. Dvorak, and P. F. Weller Roles and origins of leukocyte lipid bodies: proteomic and ultrastructural studies FASEB J, January 1, 2007; 21(1): 167 - 178. [Abstract] [Full Text] [PDF]

				M. Luo, S. M. Jones, M. Peters-Golden, and T. G. Brock Nuclear localization of 5-lipoxygenase as a determinant of leukotriene B4 synthetic capacity PNAS, October 14, 2003; 100(21): 12165 - 12170. [Abstract] [Full Text] [PDF]

				E. F. de Assis, A. R. Silva, L. F. C. Caiado, G. K. Marathe, G. A. Zimmerman, S. M. Prescott, T. M. McIntyre, P. T. Bozza, and H. C. de Castro-Faria-Neto Synergism Between Platelet-Activating Factor-Like Phospholipids and Peroxisome Proliferator-Activated Receptor {gamma} Agonists Generated During Low Density Lipoprotein Oxidation That Induces Lipid Body Formation in Leukocytes J. Immunol., August 15, 2003; 171(4): 2090 - 2098. [Abstract] [Full Text] [PDF]

				P. Pacheco, F. A. Bozza, R. N. Gomes, M. Bozza, P. F. Weller, H. C. Castro-Faria-Neto, and P. T. Bozza Lipopolysaccharide-Induced Leukocyte Lipid Body Formation In Vivo: Innate Immunity Elicited Intracellular Loci Involved in Eicosanoid Metabolism J. Immunol., December 1, 2002; 169(11): 6498 - 6506. [Abstract] [Full Text] [PDF]

				L. M. Scarfo, P. F. Weller, and H. W. Farber Induction of endothelial cell cytoplasmic lipid bodies during hypoxia Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H294 - H301. [Abstract] [Full Text] [PDF]

				W. Yu, J. Cassara, and P. F. Weller Phosphatidylinositide 3-kinase localizes to cytoplasmic lipid bodies in human polymorphonuclear leukocytes and other myeloid-derived cells Blood, February 1, 2000; 95(3): 1078 - 1085. [Abstract] [Full Text] [PDF]

				M. A. Giembycz and M. A. Lindsay Pharmacology of the Eosinophil Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340. [Abstract] [Full Text] [PDF]

				K. R. Bartemes, S. McKinney, G. J. Gleich, and H. Kita Endogenous Platelet-Activating Factor Is Critically Involved in Effector Functions of Eosinophils Stimulated with IL-5 or IgG J. Immunol., March 1, 1999; 162(5): 2982 - 2989. [Abstract] [Full Text] [PDF]

				P. T. Bozza,  W. Yu, J. F. Penrose, E. S. Morgan, A. M. Dvorak, and P. F. Weller Eosinophil Lipid Bodies: Specific, Inducible Intracellular Sites for Enhanced Eicosanoid Formation J. Exp. Med., September 15, 1997; 186(6): 909 - 920. [Abstract] [Full Text] [PDF]

				C. Bandeira-Melo, M. Phoofolo, and P. F. Weller Extranuclear Lipid Bodies, Elicited by CCR3-mediated Signaling Pathways, Are the Sites of Chemokine-enhanced Leukotriene C4 Production in Eosinophils and Basophils J. Biol. Chem., June 15, 2001; 276(25): 22779 - 22787. [Abstract] [Full Text] [PDF]