help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Order Full text via Infotrieve
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagata, N.
Right arrow Articles by Tanaka, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagata, N.
Right arrow Articles by Tanaka, K.

American Journal of Pathology, Vol 138, 183-193, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Anatomic pathway of pulmonary fluid leakage in endotoxemia induced in rats. The red blood cell packing method and its application

N Nagata, K Sueishi and K Tanaka
Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

The anatomic site of pulmonary fluid leakage in endotoxemia in rats was investigated using the red blood cell packing method and low-dose horseradish peroxidase as a tracer. To differentiate between arterioles and venules in a given section by light and electron microscopy, human red blood cells fixed with 4% paraformaldehyde were administered to the rat pulmonary arterial trunk at a pressure of 40 cm water. Fixed red blood cells were packed in the lumina of arteries, arterioles, and a few capillaries surrounding arterioles, while veins, venules, and almost all capillaries were void of red blood cells in the lumina. Fifteen minutes after the intravenous administration of 3 and 30 mg/kg of Escherichia coli endotoxin, extravascular leakage of horseradish peroxidase from venules (nonmuscular veins) was evident. Two hours after the intravenous injection of the same doses of endotoxin, some arterioles (nonmuscular arteries) and venules (non-muscular veins) showed extravascular leakage of horseradish peroxidase, while few capillaries showed this leakage. These results suggest that pulmonary fluid leakage occurs predominantly through venules in the early phase of endotoxemia (at 15 minutes), while the arterioles contribute to fluid leakage in addition to venules in the late phase of endotoxemia (at 2 hours).




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the American Society for Investigative Pathology.