| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 138, 1045-1057, Copyright © 1991 by American Society for Investigative Pathology
REGULAR ARTICLES |
GA Ferns, MA Reidy and R Ross
Department of Pathology, University of Washington, Seattle 98195.
The development of an intimal proliferative lesion after balloon catheter de-endothelialization was studied in congenitally athymic nude rats lacking T lymphocytes. Significant intimal thickening was observed in both the homozygous (nu/nu) and euthymic heterozygous (nu/+) animals 6 days after injury, which increased further after 10 days. There was no significant difference in mean intimal:medial cross-sectional area between the nu/nu and nu/+ animals at either time. Approximately 1% of the cells in the neointima of both groups of animals were leukocytes (OX-1 positive); 0.7% were macrophages (ED-1 positive). In neither nu/nu nor nu/+ animals did T lymphocytes (OX-19-positive cells) constitute more than 0.1% of the neointimal cell population. These data suggest that T lymphocytes do not play a significant role in the accumulation of neointimal cells. The presence of macrophages within the lesions raises the possibility that they may be involved in the recruitment and proliferation of smooth muscle cells. In vitro characterization of nu/nu carotid medial smooth muscle cells demonstrated approximately 500,000 binding sites for platelet-derived growth factor (PDGF)-BB and few PDGF-AA binding sites (less than 10,000). The mitogenic and chemotactic responses of these cells to the three dimeric forms of PDGF correlated with this receptor subunit distribution. Platelet-derived growth factor accounted for approximately 50% of the mitogenic activity of a rat platelet releasate. Platelet-derived growth factor-BB and PDGF-AB were both potent chemotactic agents for the nude rat carotid smooth muscle cells with a peak response at approximately 10 ng/ml. In contrast, PDGF-AA, transforming growth factor beta, and basic fibroblast growth factor were only weak chemoattractants for these cells.
This article has been cited by other articles:
 |
|
 |
|
  C. E. Chadjichristos, C. M. Matter, I. Roth, E. Sutter, G. Pelli, T. F. Luscher, M. Chanson, and B. R. Kwak Reduced Connexin43 Expression Limits Neointima Formation After Balloon Distension Injury in Hypercholesterolemic Mice Circulation, June 20, 2006; 113(24): 2835 - 2843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tedgui and Z. Mallat Cytokines in Atherosclerosis: Pathogenic and Regulatory Pathways Physiol Rev, April 1, 2006; 86(2): 515 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Md. R. Abid, K. Yano, S. Guo, V. I. Patel, G. Shrikhande, K. C. Spokes, C. Ferran, and W. C. Aird Forkhead Transcription Factors Inhibit Vascular Smooth Muscle Cell Proliferation and Neointimal Hyperplasia J. Biol. Chem., August 19, 2005; 280(33): 29864 - 29873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Satonaka, E. Suzuki, H. Nishimatsu, S. Oba, R. Takeda, A. Goto, M. Omata, T. Fujita, R. Nagai, and Y. Hirata Calcineurin Promotes the Expression of Monocyte Chemoattractant Protein-1 in Vascular Myocytes and Mediates Vascular Inflammation Circ. Res., March 19, 2004; 94(5): 693 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Seye, Q. Kong, L. Erb, R. C. Garrad, B. Krugh, M. Wang, J. T. Turner, M. Sturek, F. A. Gonzalez, and G. A. Weisman Functional P2Y2 Nucleotide Receptors Mediate Uridine 5'-Triphosphate-Induced Intimal Hyperplasia in Collared Rabbit Carotid Arteries Circulation, November 19, 2002; 106(21): 2720 - 2726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Zhu, C. A. Reardon, G. S. Getz, and D. Y. Hui Both Apolipoprotein E and Immune Deficiency Exacerbate Neointimal Hyperplasia After Vascular Injury in Mice Arterioscler. Thromb. Vasc. Biol., March 1, 2002; 22(3): 450 - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. O. Andersen, B. F. Hansen, P. Holm, S. Stender, and B. G. Nordestgaard Effect of Cyclosporine on Arterial Balloon Injury Lesions in Cholesterol-Clamped Rabbits : T Lymphocyte–Mediated Immune Responses Not Involved in Balloon Injury–Induced Neointimal Proliferation Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1687 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Furukawa, A. Matsumori, N. Ohashi, T. Shioi, K. Ono, A. Harada, K. Matsushima, and S. Sasayama Anti–Monocyte Chemoattractant Protein-1/Monocyte Chemotactic and Activating Factor Antibody Inhibits Neointimal Hyperplasia in Injured Rat Carotid Arteries Circ. Res., February 19, 1999; 84(3): 306 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Forough, H. Lea, B. Starcher, E. Allaire, M. Clowes, D. Hasenstab, and A. W. Clowes Metalloproteinase Blockade by Local Overexpression of TIMP-1 Increases Elastin Accumulation in Rat Carotid Artery Intima Arterioscler. Thromb. Vasc. Biol., May 1, 1998; 18(5): 803 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Seye, A.-P. Gadeau, D. Daret, F. Dupuch, P. Alzieu, L. Capron, and C. Desgranges Overexpression of the P2Y2 Purinoceptor in Intimal Lesions of the Rat Aorta Arterioscler. Thromb. Vasc. Biol., December 1, 1997; 17(12): 3602 - 3610. [Abstract] [Full Text]
|
|
|
|
|
|
H.-I Yeh, F. Lupu, E. Dupont, and N. J. Severs Upregulation of Connexin43 Gap Junctions Between Smooth Muscle Cells After Balloon Catheter Injury in the Rat Carotid Artery Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 3174 - 3184. [Abstract] [Full Text]
|
|
|
|
|
|
L. G. Borges, R. A. Seifert, F. J. Grant, C. E. Hart, C. M. Disteche, S. Edelhoff, F. F. Solca, M. A. Lieberman, V. Lindner, E. H. Fischer, et al. Cloning and Characterization of Rat Density-Enhanced Phosphatase-1, a Protein Tyrosine Phosphatase Expressed by Vascular Cells Circ. Res., September 1, 1996; 79(3): 570 - 580. [Abstract] [Full Text]
|
|
|
|
|
|
B. E. Rolfe, J. H. Campbell, N. J. Smith, M. W. Cheong, and G. R. Campbell T Lymphocytes Affect Smooth Muscle Cell Phenotype and Proliferation Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1204 - 1210. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. V. Petrik, H. A. Gelabert, W. S. Moore, W. Quinones-Baldrich, and M. M. Law Cigarette Smoking Accelerates Carotid Artery Intimal Hyperplasia in a Dose-Dependent Manner Stroke, August 1, 1995; 26(8): 1409 - 1414. [Abstract] [Full Text]
|
|
|
|
|
|
V. Lindner, C. M. Giachelli, S. M. Schwartz, and M. A. Reidy A Subpopulation of Smooth Muscle Cells in Injured Rat Arteries Expresses Platelet-Derived Growth Factor–B Chain mRNA Circ. Res., June 1, 1995; 76(6): 951 - 957. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. A. Parish, E. A. Grossi, F. G. Baumann, T. Asai, D. B. Rifkin, S. B. Colvin, and A. C. Galloway Effects of a Single Administration of Fibroblast Growth Factor on Vascular Wall Reaction to Injury Ann. Thorac. Surg., April 1, 1995; 59(4): 948 - 954. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Ferns, E. Raines, K. Sprugel, A. Motani, M. Reidy, and R Ross Inhibition of neointimal smooth muscle accumulation after angioplasty by an antibody to PDGF Science, September 6, 1991; 253(5024): 1129 - 1132. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
