This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Matsubara, S. Articles by Kambara, T. Search for Related Content PubMed PubMed Citation Articles by Matsubara, S. Articles by Kambara, T.

American Journal of Pathology, Vol 138, 1279-1291, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Complement C4-derived monocyte-directed chemotaxis-inhibitory factor. A molecular mechanism to cause polymorphonuclear leukocyte-predominant infiltration in rheumatoid arthritis synovial cavities

S Matsubara, T Yamamoto, T Tsuruta, K Takagi and T Kambara
Department of Orthopaedic Surgery, Kumamoto University Medical School, Japan.

To reveal the mechanism of the lesser infiltration of monocytes in synovial cavities with rheumatoid arthritis despite the presence of chronic inflammation, the synovial fluid from 15 rheumatoid arthritis patients was analyzed with respect to leukocyte chemotaxis. The synovial fluid possessed strong chemotactic activity to polymorphonuclear leukocytes but rather suppressed one to monocytes. The synovial fluid contained two different inhibitory activities in monocyte chemotaxis. One, which also suppressed polymorphonuclear leukocyte chemotaxis, was identified as alpha 1 protease inhibitor. The other, with molecular weight of 8 kd, possessed the specificity to monocytes and shared the antigenicity with complement C4 but not with C3 or C5. A similar inhibitor was generated in normal human plasma when the classical pathway of the complement system was initiated with aggregated human IgG, while it was not when alternative pathway was initiated with zymosan. The small size factor in the synovial fluid, apparently derived from C4, seemed to be a cyto-directed factor that might block an early part of signal transduction system of monocytes in the chemotaxis. After removal of the small-size inhibitor, the synovial fluid exhibited chemotactic ability to monocytes. Therefore the apparent C4-derived factor might play a key role in the polymorphonuclear leukocyte-predominant infiltration in the synovial fluid of rheumatoid arthritis.

This article has been cited by other articles:

				Y. Oda, K. Tokita, Y. Ota, Y. Li, K. Taniguchi, N. Nishino, K. Takagi, T. Yamamoto, and H. Nishiura Agonistic and Antagonistic Effects of C5a-Chimera Bearing S19 Ribosomal Protein Tail Portion on the C5a Receptor of Monocytes and Neutrophils, Respectively J. Biochem., September 1, 2008; 144(3): 371 - 381. [Abstract] [Full Text] [PDF]

				A. Shrestha, L. Shi, S. Tanase, M. Tsukamoto, N. Nishino, K. Tokita, and T. Yamamoto Bacterial Chaperone Protein, Skp, Induces Leukocyte Chemotaxis via C5a Receptor Am. J. Pathol., March 1, 2004; 164(3): 763 - 772. [Abstract] [Full Text] [PDF]

				A. Shrestha, M. Shiokawa, T. Nishimura, H. Nishiura, Y. Tanaka, N. Nishino, Y. Shibuya, and T. Yamamoto Switch Moiety in Agonist/Antagonist Dual Effect of S19 Ribosomal Protein Dimer on Leukocyte Chemotactic C5a Receptor Am. J. Pathol., April 1, 2003; 162(4): 1381 - 1388. [Abstract] [Full Text] [PDF]

				Y. Shibuya, M. Shiokawa, H. Nishiura, T. Nishimura, N. Nishino, H. Okabe, K. Takagi, and T. Yamamoto Identification of Receptor-Binding Sites of Monocyte Chemotactic S19 Ribosomal Protein Dimer Am. J. Pathol., December 1, 2001; 159(6): 2293 - 2301. [Abstract] [Full Text] [PDF]

				H. Nishiura, Y. Shibuya, S. Matsubara, S. Tanase, T. Kambara, and T. Yamamoto Monocyte Chemotactic Factor in Rheumatoid Arthritis Synovial Tissue J. Biol. Chem., January 12, 1996; 271(2): 878 - 882. [Abstract] [Full Text] [PDF]