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American Journal of Pathology, Vol 138, 1299-1305, Copyright © 1991 by American Society for Investigative Pathology

REGULAR ARTICLES

Interleukin-1 mRNA-expressing macrophages in human chronically inflamed gingival tissues

Y Matsuki, T Yamamoto and K Hara
Department of Periodontology, Niigata University School of Dentistry, Japan.

Interleukin-1 (IL-1)-producing cells in inflamed gingival tissues obtained from patients with periodontitis were examined by combined immunohistochemistry and in situ hybridization. Macrophages, T cells, B cells, and endothelial cells were visualized in the lesions by the immunoperoxidase method using specific antibodies to each cell type. Subsequent in situ hybridization using 35S-labeled synthetic oligonucleotide probes complementary to human IL-1 alpha and IL-1 beta mRNA showed IL-1 transcripts in macrophages predominantly but not in T cells or B cells. Neither fibroblasts nor keratinocytes contained the IL-1 message to any significant extent. Endothelial cells also were essentially negative for IL-1 messages. These findings indicate that IL- 1 is produced principally by macrophages in inflamed gingival tissues of humans.




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Copyright © 1991 by the American Society for Investigative Pathology.